Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid

Manuel Krauskopf, Alejandro Araya, Simon Litvak

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

1. 1. The isolation of tRNA from the liver of the shark Mustelus mento is described. The prcedure involves phenol extraction, chromatography on DEAE-cellulose and isopropanol fractionation. The obtained fractions were analyzed by plyacrylamide gel electrophoresis. 2. 2. The tRNA was shown to have an intact 3' end and to accept the four amino acids tested with homologous aminoacyl-tRNA synthetases. 3. 3. Analytical centrifuge analysis gave a sedimentation coefficient of S20,w=4·01. 4. 4. Thermal denaturation profiles were compared to other tRNAs.

Original languageEnglish
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume48
Issue number4
DOIs
Publication statusPublished - 15 Aug 1974

Fingerprint

Fish Proteins
Sharks
Transfer RNA
Liver
Amino Acyl-tRNA Synthetases
DEAE-Cellulose Chromatography
2-Propanol
Phenol
Electrophoresis
Hot Temperature
Gels
Amino Acids

Keywords

  • Mustelus mento
  • poikilotherm
  • protein synthesis
  • shark
  • tRNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{df5df3d0a1f3483c9b7b287b86fec535,
title = "Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid",
abstract = "1. 1. The isolation of tRNA from the liver of the shark Mustelus mento is described. The prcedure involves phenol extraction, chromatography on DEAE-cellulose and isopropanol fractionation. The obtained fractions were analyzed by plyacrylamide gel electrophoresis. 2. 2. The tRNA was shown to have an intact 3' end and to accept the four amino acids tested with homologous aminoacyl-tRNA synthetases. 3. 3. Analytical centrifuge analysis gave a sedimentation coefficient of S20,w=4·01. 4. 4. Thermal denaturation profiles were compared to other tRNAs.",
keywords = "Mustelus mento, poikilotherm, protein synthesis, shark, tRNA",
author = "Manuel Krauskopf and Alejandro Araya and Simon Litvak",
year = "1974",
month = "8",
day = "15",
doi = "10.1016/0305-0491(74)90142-4",
language = "English",
volume = "48",
journal = "Comparative biochemistry and physiology. B, Comparative biochemistry",
issn = "1096-4959",
publisher = "Elsevier Inc.",
number = "4",

}

Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid. / Krauskopf, Manuel; Araya, Alejandro; Litvak, Simon.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 48, No. 4, 15.08.1974.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid

AU - Krauskopf, Manuel

AU - Araya, Alejandro

AU - Litvak, Simon

PY - 1974/8/15

Y1 - 1974/8/15

N2 - 1. 1. The isolation of tRNA from the liver of the shark Mustelus mento is described. The prcedure involves phenol extraction, chromatography on DEAE-cellulose and isopropanol fractionation. The obtained fractions were analyzed by plyacrylamide gel electrophoresis. 2. 2. The tRNA was shown to have an intact 3' end and to accept the four amino acids tested with homologous aminoacyl-tRNA synthetases. 3. 3. Analytical centrifuge analysis gave a sedimentation coefficient of S20,w=4·01. 4. 4. Thermal denaturation profiles were compared to other tRNAs.

AB - 1. 1. The isolation of tRNA from the liver of the shark Mustelus mento is described. The prcedure involves phenol extraction, chromatography on DEAE-cellulose and isopropanol fractionation. The obtained fractions were analyzed by plyacrylamide gel electrophoresis. 2. 2. The tRNA was shown to have an intact 3' end and to accept the four amino acids tested with homologous aminoacyl-tRNA synthetases. 3. 3. Analytical centrifuge analysis gave a sedimentation coefficient of S20,w=4·01. 4. 4. Thermal denaturation profiles were compared to other tRNAs.

KW - Mustelus mento

KW - poikilotherm

KW - protein synthesis

KW - shark

KW - tRNA

UR - http://www.scopus.com/inward/record.url?scp=0016211462&partnerID=8YFLogxK

U2 - 10.1016/0305-0491(74)90142-4

DO - 10.1016/0305-0491(74)90142-4

M3 - Article

VL - 48

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

JF - Comparative biochemistry and physiology. B, Comparative biochemistry

SN - 1096-4959

IS - 4

ER -