Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid

Manuel Krauskopf, Alejandro Araya, Simon Litvak

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

1. 1. The isolation of tRNA from the liver of the shark Mustelus mento is described. The prcedure involves phenol extraction, chromatography on DEAE-cellulose and isopropanol fractionation. The obtained fractions were analyzed by plyacrylamide gel electrophoresis. 2. 2. The tRNA was shown to have an intact 3' end and to accept the four amino acids tested with homologous aminoacyl-tRNA synthetases. 3. 3. Analytical centrifuge analysis gave a sedimentation coefficient of S20,w=4·01. 4. 4. Thermal denaturation profiles were compared to other tRNAs.

Original languageEnglish
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume48
Issue number4
DOIs
Publication statusPublished - 15 Aug 1974

Keywords

  • Mustelus mento
  • poikilotherm
  • protein synthesis
  • shark
  • tRNA

ASJC Scopus subject areas

  • Medicine(all)

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