1. 1. The isolation of tRNA from the liver of the shark Mustelus mento is described. The prcedure involves phenol extraction, chromatography on DEAE-cellulose and isopropanol fractionation. The obtained fractions were analyzed by plyacrylamide gel electrophoresis. 2. 2. The tRNA was shown to have an intact 3' end and to accept the four amino acids tested with homologous aminoacyl-tRNA synthetases. 3. 3. Analytical centrifuge analysis gave a sedimentation coefficient of S20,w=4·01. 4. 4. Thermal denaturation profiles were compared to other tRNAs.
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|Publication status||Published - 15 Aug 1974|
- Mustelus mento
- protein synthesis
ASJC Scopus subject areas