Structure and processing of yeast precursor tRNAs containing intervening sequences

P. Z. O'Farrell; B. Cordell; P. Valenzuela; W. J. Rutter; H. M. Goodman

doi:10.1038/274438a0

Structure and processing of yeast precursor tRNAs containing intervening sequences

P. Z. O'Farrell, B. Cordell, P. Valenzuela, W. J. Rutter, H. M. Goodman

Life Sciences School

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73 Citations (Scopus)

Abstract

We have isolated a precursor of yeast tRNA^Tyr and shown that it contains an intervening sequence identical to that found in the gene for tRNA^Tyr. The conformation of pre-tRNA^Tyr is similar to that of mature tRNA^Tyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S₁ nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNA^Tyr into mature tRNA^Tyr.

Original language	English
Pages (from-to)	438-445
Number of pages	8
Journal	Nature
Volume	274
Issue number	5670
DOIs	https://doi.org/10.1038/274438a0
Publication status	Published - 1 Dec 1978

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title = "Structure and processing of yeast precursor tRNAs containing intervening sequences",

abstract = "We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.",

author = "O'Farrell, {P. Z.} and B. Cordell and P. Valenzuela and Rutter, {W. J.} and Goodman, {H. M.}",

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T1 - Structure and processing of yeast precursor tRNAs containing intervening sequences

AU - O'Farrell, P. Z.

AU - Cordell, B.

AU - Valenzuela, P.

AU - Rutter, W. J.

AU - Goodman, H. M.

PY - 1978/12/1

Y1 - 1978/12/1

N2 - We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.

AB - We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.

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DO - 10.1038/274438a0

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SN - 0028-0836

VL - 274

SP - 438

EP - 445

JO - Nature

JF - Nature

IS - 5670

ER -

Structure and processing of yeast precursor tRNAs containing intervening sequences

Abstract

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