TY - JOUR
T1 - Stocking density induces differential expression of immune-related genes in skeletal muscle and head kidney of fine flounder (Paralichthys adspersus)
AU - Valenzuela, Cristián A.
AU - Escobar-Aguirre, Sebastián
AU - Zuloaga, Rodrigo
AU - Vera-Tobar, Tamara
AU - Mercado, Luis
AU - Björnsson, Björn Thrandur
AU - Valdés, Juan A.
AU - Molina, Alfredo
N1 - Funding Information:
This work was supported by Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) Grant 1171307 (to A. Molina); Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (FONDAP) Grants INCAR 15110027 (to J. Valdés and A. Molina). Sebastián Escobar-Aguirre was supported by Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) postdoctoral grant, Project 3160370 .
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Immunity can be modulated by different internal and external factors, being stress one of the most important. However, the stress effects on the immunocompetence of the skeletal muscle has not been studied in detail in earlier vertebrates. Here, we examine the effect of chronic (4 and 7 weeks) crowding stress on the immunocompetence of skeletal muscle and head kidney in the fine flounder (Paralichthys adspersus). Corticosteroid receptor transcript levels and their target genes; pro-inflammatory cytokines, and Toll-, NOD-, and RIG-like receptors were quantified by qPCR. The results indicate that chronic stress down-regulates the expression of these genes in muscle, compromising skeletal muscle immunocompetence, while the expression of these genes is upregulated in head kidney after seven weeks of crowding stress. The data suggests that chronic stress modulates the expression of these immune-related genes in a tissue-specific manner.
AB - Immunity can be modulated by different internal and external factors, being stress one of the most important. However, the stress effects on the immunocompetence of the skeletal muscle has not been studied in detail in earlier vertebrates. Here, we examine the effect of chronic (4 and 7 weeks) crowding stress on the immunocompetence of skeletal muscle and head kidney in the fine flounder (Paralichthys adspersus). Corticosteroid receptor transcript levels and their target genes; pro-inflammatory cytokines, and Toll-, NOD-, and RIG-like receptors were quantified by qPCR. The results indicate that chronic stress down-regulates the expression of these genes in muscle, compromising skeletal muscle immunocompetence, while the expression of these genes is upregulated in head kidney after seven weeks of crowding stress. The data suggests that chronic stress modulates the expression of these immune-related genes in a tissue-specific manner.
KW - Crowding stress
KW - Fish
KW - Immune receptors
KW - Stress response
UR - http://www.scopus.com/inward/record.url?scp=85062829765&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2019.03.004
DO - 10.1016/j.vetimm.2019.03.004
M3 - Article
AN - SCOPUS:85062829765
SN - 0165-2427
VL - 210
SP - 23
EP - 27
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
ER -