Specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum

G. León, M. A. Martinez, J. P. Etchegaray, M. I. Vera, J. Figueroa, M. Krauskopf

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

To obtain specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum, a discriminatory recombinant DNA library was constructed using selective fragments of the bacterial genome. Three renibacterial clones, pMAM29, pMAM46 and pMAM77, containing 149, 73, and 154 bp respectively, were isolated and characterized. The specificity of the probes was confirmed by dot-blot and Southern hybridization analyses. Bacterial hybridization experiments revealed that pMAM29 discriminates the R. salmoninarum genome from that of other fish pathogens such as Aeromonas salmonicida, Yersinia ruckeri, Flexibacter columnaris, Lactobacillus piscicola, Vibrio ordalii, Vibrio anguillarum and Aeromonas hydrophila. Thus, this probe may provide a new means to diagnose bacterial kidney disease in asymptomatic fish and ova.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalWorld Journal of Microbiology & Biotechnology
Volume10
Issue number2
DOIs
Publication statusPublished - 1 Mar 1994

Keywords

  • Bacterial kidney disease
  • fish pathogens
  • probes
  • Renibacterium salmoninarum
  • salmon

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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