Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC33020 strain

Abderrahmane Bengrine, Nicolas Guiliani, Corinne Appia-Ayme, Eugenia Jedlicki, David S. Holmes, Marc Chippaux, V. Bonnefoy

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

The periplasmic blue copper protein rusticyanin is thought to play an important role in iron oxidation by Thiobacillus ferrooxidans. We present the sequence of the gene, rus, encoding rusticyanin together with about 1.4 kb of upstream and 0.3 kb of downstream DNA. The rus gene is unique to T. ferrooxidans. Evidence is presented that it is the last gene of an operon and that it can be transcribed from its own promoter. In ATCC33020 strain, rusticyanin is synthesized in ferrous iron but also in sulfur growth conditions suggesting that it could play a role in both energetic metabolisms. The rus gene transcribed from a vector promoter in Escherichia coli leads to the production of a processed aporusticyanin in the periplasmic space, indicating that its signal sequence is correctly recognized by the secretion machinery and the signal peptidase of E. coli.

Original languageEnglish
Pages (from-to)99-112
Number of pages14
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1443
Issue number1-2
DOIs
Publication statusPublished - 26 Nov 1998

Keywords

  • (Thiobacillus ferrooxidans)
  • Copper protein
  • Electron transport
  • Iron oxidation
  • Rusticyanin

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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