TY - JOUR
T1 - Selective changes in methionine tRNA species during development of the posterior silkglands of Bombyx mori
AU - Araya, A.
AU - Krauskopf, M.
AU - Siddiqui, M. A Q
PY - 1975/12/1
Y1 - 1975/12/1
N2 - Transfer RNA with methionine acceptor activity isolated from two distinct physiological stages of the developing posterior silkgland of the silkworm, Bombyx mori, was examined. The tRNA from both stages could be fractionated on benzoylated DEAE-cellulose colum into two iso-accepting species, tRNA1
Met and tRNA2
Met. The molar quantity per gland of tRNA1
Met species, which was also formylatable with the E. coli enzymes, increased twelve-fold as the gland differentiates to produce a large amount of a single protein, silk-fibroin. Since methionine is not a part of silk-fibroin, the preferential increase in tRNA1
Met content would reflect the increased biological activity and the rapid rate of protein synthesis during the terminal differentiation of posterior silkgland.
AB - Transfer RNA with methionine acceptor activity isolated from two distinct physiological stages of the developing posterior silkgland of the silkworm, Bombyx mori, was examined. The tRNA from both stages could be fractionated on benzoylated DEAE-cellulose colum into two iso-accepting species, tRNA1
Met and tRNA2
Met. The molar quantity per gland of tRNA1
Met species, which was also formylatable with the E. coli enzymes, increased twelve-fold as the gland differentiates to produce a large amount of a single protein, silk-fibroin. Since methionine is not a part of silk-fibroin, the preferential increase in tRNA1
Met content would reflect the increased biological activity and the rapid rate of protein synthesis during the terminal differentiation of posterior silkgland.
UR - http://www.scopus.com/inward/record.url?scp=0016836622&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(75)90765-2
DO - 10.1016/0006-291X(75)90765-2
M3 - Article
C2 - 1201082
AN - SCOPUS:0016836622
VL - 67
SP - 924
EP - 934
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -