Transfer RNA with methionine acceptor activity isolated from two distinct physiological stages of the developing posterior silkgland of the silkworm, Bombyx mori, was examined. The tRNA from both stages could be fractionated on benzoylated DEAE-cellulose colum into two iso-accepting species, tRNA1 Met and tRNA2 Met. The molar quantity per gland of tRNA1 Met species, which was also formylatable with the E. coli enzymes, increased twelve-fold as the gland differentiates to produce a large amount of a single protein, silk-fibroin. Since methionine is not a part of silk-fibroin, the preferential increase in tRNA1 Met content would reflect the increased biological activity and the rapid rate of protein synthesis during the terminal differentiation of posterior silkgland.
|Number of pages||11|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 1 Dec 1975|
ASJC Scopus subject areas
- Molecular Biology