Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA

Renato Chávez, Claudio Navarro, Inés Calderón, Alessandra Peirano, Paulina Bull, Jaime Eyzaguirre

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing.

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalFEMS Microbiology Letters
Volume212
Issue number2
DOIs
Publication statusPublished - 2 Jul 2002

Keywords

  • Heterologous gene expression
  • Penicillium purpurogenum
  • Saccharomyces cerevisiae
  • Xylanases

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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