Salmonella typhi Ty2 OmpC porin induces bactericidal activity on U937 monocytes

Luz P. Blanco, Cecilia S. Toro, Jaime M. Romero, Carlos A. Santiviago, Guido C. Mora

Research output: Contribution to journalComment/debatepeer-review

8 Citations (Scopus)

Abstract

The immunogenic effect of Salmonella typhi OmpC porin during typhoid fever in humans was evaluated in vitro. Peripheral blood mononuclear cells from 17 patients were challenged with outer membrane preparations from Escherichia coli UH302 and UH302/pSTP2K2 strains, both lacking E. coli OmpF and OmpC porins, although UH302/pSTP2K2 expressed a plasmid-encoded S. typhi Ty2 OmpC. The mononuclear cell supernatants, immunized in vitro with OmpC antigen, derived from 10 out of 17 patients activated U937 bactericidal capacity. In contrast, the supernatants from the immunization with outer membrane preparation lacking S. typhi Ty2 OmpC induced a significantly reduced bactericidal capacity of U937 cells. This procedure should prove useful for in vitro characterization of cellular immunogens from exclusive human pathogens.

Original languageEnglish
Pages (from-to)999-1003
Number of pages5
JournalMicrobiology and Immunology
Volume41
Issue number12
DOIs
Publication statusPublished - 1997

Keywords

  • Macrophage activation
  • Mononuclear cells
  • Salmonella typhi OmpC
  • Typhoid patients

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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