Salmonella typhi Ty2 OmpC porin induces bactericidal activity on U937 monocytes

Luz P. Blanco; Cecilia S. Toro; Jaime M. Romero; Carlos A. Santiviago; Guido C. Mora

doi:10.1111/j.1348-0421.1997.tb01960.x

Salmonella typhi Ty2 OmpC porin induces bactericidal activity on U937 monocytes

Luz P. Blanco, Cecilia S. Toro, Jaime M. Romero, Carlos A. Santiviago, Guido C. Mora

Medicine School

Research output: Contribution to journal › Comment/debate › peer-review

8 Citations (Scopus)

Abstract

The immunogenic effect of Salmonella typhi OmpC porin during typhoid fever in humans was evaluated in vitro. Peripheral blood mononuclear cells from 17 patients were challenged with outer membrane preparations from Escherichia coli UH302 and UH302/pSTP2K2 strains, both lacking E. coli OmpF and OmpC porins, although UH302/pSTP2K2 expressed a plasmid-encoded S. typhi Ty2 OmpC. The mononuclear cell supernatants, immunized in vitro with OmpC antigen, derived from 10 out of 17 patients activated U937 bactericidal capacity. In contrast, the supernatants from the immunization with outer membrane preparation lacking S. typhi Ty2 OmpC induced a significantly reduced bactericidal capacity of U937 cells. This procedure should prove useful for in vitro characterization of cellular immunogens from exclusive human pathogens.

Original language	English
Pages (from-to)	999-1003
Number of pages	5
Journal	Microbiology and Immunology
Volume	41
Issue number	12
DOIs	https://doi.org/10.1111/j.1348-0421.1997.tb01960.x
Publication status	Published - 1997

Keywords

Macrophage activation
Mononuclear cells
Salmonella typhi OmpC
Typhoid patients

ASJC Scopus subject areas

Microbiology
Immunology
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1348-0421.1997.tb01960.x

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title = "Salmonella typhi Ty2 OmpC porin induces bactericidal activity on U937 monocytes",

abstract = "The immunogenic effect of Salmonella typhi OmpC porin during typhoid fever in humans was evaluated in vitro. Peripheral blood mononuclear cells from 17 patients were challenged with outer membrane preparations from Escherichia coli UH302 and UH302/pSTP2K2 strains, both lacking E. coli OmpF and OmpC porins, although UH302/pSTP2K2 expressed a plasmid-encoded S. typhi Ty2 OmpC. The mononuclear cell supernatants, immunized in vitro with OmpC antigen, derived from 10 out of 17 patients activated U937 bactericidal capacity. In contrast, the supernatants from the immunization with outer membrane preparation lacking S. typhi Ty2 OmpC induced a significantly reduced bactericidal capacity of U937 cells. This procedure should prove useful for in vitro characterization of cellular immunogens from exclusive human pathogens.",

keywords = "Macrophage activation, Mononuclear cells, Salmonella typhi OmpC, Typhoid patients",

author = "Blanco, {Luz P.} and Toro, {Cecilia S.} and Romero, {Jaime M.} and Santiviago, {Carlos A.} and Mora, {Guido C.}",

year = "1997",

doi = "10.1111/j.1348-0421.1997.tb01960.x",

language = "English",

volume = "41",

pages = "999--1003",

journal = "Microbiology and Immunology",

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TY - JOUR

T1 - Salmonella typhi Ty2 OmpC porin induces bactericidal activity on U937 monocytes

AU - Blanco, Luz P.

AU - Toro, Cecilia S.

AU - Romero, Jaime M.

AU - Santiviago, Carlos A.

AU - Mora, Guido C.

PY - 1997

Y1 - 1997

N2 - The immunogenic effect of Salmonella typhi OmpC porin during typhoid fever in humans was evaluated in vitro. Peripheral blood mononuclear cells from 17 patients were challenged with outer membrane preparations from Escherichia coli UH302 and UH302/pSTP2K2 strains, both lacking E. coli OmpF and OmpC porins, although UH302/pSTP2K2 expressed a plasmid-encoded S. typhi Ty2 OmpC. The mononuclear cell supernatants, immunized in vitro with OmpC antigen, derived from 10 out of 17 patients activated U937 bactericidal capacity. In contrast, the supernatants from the immunization with outer membrane preparation lacking S. typhi Ty2 OmpC induced a significantly reduced bactericidal capacity of U937 cells. This procedure should prove useful for in vitro characterization of cellular immunogens from exclusive human pathogens.

AB - The immunogenic effect of Salmonella typhi OmpC porin during typhoid fever in humans was evaluated in vitro. Peripheral blood mononuclear cells from 17 patients were challenged with outer membrane preparations from Escherichia coli UH302 and UH302/pSTP2K2 strains, both lacking E. coli OmpF and OmpC porins, although UH302/pSTP2K2 expressed a plasmid-encoded S. typhi Ty2 OmpC. The mononuclear cell supernatants, immunized in vitro with OmpC antigen, derived from 10 out of 17 patients activated U937 bactericidal capacity. In contrast, the supernatants from the immunization with outer membrane preparation lacking S. typhi Ty2 OmpC induced a significantly reduced bactericidal capacity of U937 cells. This procedure should prove useful for in vitro characterization of cellular immunogens from exclusive human pathogens.

KW - Macrophage activation

KW - Mononuclear cells

KW - Salmonella typhi OmpC

KW - Typhoid patients

UR - http://www.scopus.com/inward/record.url?scp=0031446239&partnerID=8YFLogxK

U2 - 10.1111/j.1348-0421.1997.tb01960.x

DO - 10.1111/j.1348-0421.1997.tb01960.x

M3 - Comment/debate

C2 - 9492186

AN - SCOPUS:0031446239

SN - 0385-5600

VL - 41

SP - 999

EP - 1003

JO - Microbiology and Immunology

JF - Microbiology and Immunology

IS - 12

ER -

Salmonella typhi Ty2 OmpC porin induces bactericidal activity on U937 monocytes

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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