Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin

P. Guerrero, B. Collao, R. Álvarez, H. Salinas, E. H. Morales, I. L. Calderón, C. P. Saavedra, F. Gil

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.

Original languageEnglish
Pages (from-to)2049-2057
Number of pages9
JournalMicrobiology (United Kingdom)
Volume159
Issue numberPART 10
DOIs
Publication statusPublished - Oct 2013

ASJC Scopus subject areas

  • Microbiology

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