@article{426eb2bcdc574015a8c9b937a22f81f7,
title = "Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase",
abstract = "Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.",
keywords = "Epigenetics, Mesenchymal stem cells (MSCs), Osteoblasts, Umbilical cord",
author = "Francisco Bustos and Hugo Sep{\'u}lveda and Prieto, {Catalina P.} and Margarita Carrasco and Lorena D{\'i}az and Jos{\'e} Palma and Jos{\'e} Lattus and Mart{\'i}n Montecino and Ver{\'o}nica Palma",
note = "Funding Information: We thank Dr. Maria Rosa Bono for flow citometry expertise and MD fellows and personnel of Hospital Luis Tisn{\'e} Brousse for umbilical cord collection, especially Drs. S. Aedo, A. Sep{\'u}l-veda, C. Berr{\'i}os, F. G{\'a}lvez, and M. Acevedo. This work was supported by FONDAP CRG (Center for Genome Regulation) (15090007, to M.M. and V.P.), FONDECYT (1130706, to M.M.), FONDECYT(P) (3140368, to C.P.), and FONDEF (D09E1047, to V.P.), and CONICYT doctoral fellowship (to H.S.). F.B. is currently affiliated with the Medical Research Council, Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dundee, U.K. Funding Information: We thank Dr. Maria Rosa Bono for flow citometry expertise and MD fellows and personnel of Hospital Luis Tisn? Brousse for umbilical cord collection, especially Drs. S. Aedo, A. Sep?lveda, C. Berr?os, F. G?lvez, and M. Acevedo. This work was supported by FONDAP CRG (Center for Genome Regulation) (15090007, to M.M. and V.P.), FONDECYT (1130706, to M.M.), FONDECYT(P) (3140368, to C.P.), and FONDEF (D09E1047, to V.P.), and CONICYT doctoral fellowship (to H.S.). F.B. is currently affiliated with the Medical Research Council, Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dundee, U.K. Publisher Copyright: {\textcopyright} 2017 AlphaMed Press",
year = "2017",
doi = "10.1002/stem.2704",
language = "English",
volume = "35",
pages = "2430--2441",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "Wiley-Blackwell",
number = "12",
}