Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs

G. I. Bell, L. J. DeGennaro, D. H. Gelfand, R. J. Bishop, P. Valenzuela, W. J. Rutter

Research output: Contribution to journalArticlepeer-review

141 Citations (Scopus)

Abstract

The organization of the ribosomal DNA repeating unit from Saccharomyces cerevisiae has been analyzed. A cloned ribosomal DNA repeating unit has been mapped with the restriction enzymes Xma I, Kpn I, HindIII, Xba I, Bgl I + II, and Eco RI. The locations of the sequences which code for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs have been determined by hybridization of the purified RNA species with restriction endonuclease generated fragments of the repeating unit. The position of the 5.8 S ribosomal DNA sequences within the repeat was also established by sequencing the DNA which codes for 83 nucleotides at the 5' end of 5.8 S ribosomal RNA. The polarity of the 35 S ribosomal RNA precursor has been established by a combination of hybridization analysis and DNA sequence determination and is 5'-18 S, 5.8 S, 25 S-3'.

Original languageEnglish
Pages (from-to)8118-8125
Number of pages8
JournalJournal of Biological Chemistry
Volume252
Issue number22
Publication statusPublished - 1977

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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