Rhodococcus navarretei sp. nov. and Pseudarthrobacter quantipunctorum sp. nov., two novel species with the ability to biosynthesize fluorescent nanoparticles, isolated from soil samples at Union Glacier, Antarctica

Valentina Carrasco, Matías Vargas-Reyes, Sebastián Lagos-Moraga, Claudio Dietz-Vargas, Daniela Allendes-Ormazábal, Juan Meza-Inzunza, Fernanda Rojas-Morales, Sebastián Durán-Villegas, Felipe Valenzuela-Ibaceta, Ma Ángeles Cabrera, José M. Pérez-Donoso

Research output: Contribution to journalArticlepeer-review

Abstract

Two Gram-stain-positive bacterial strains, EXRC-4A-4T and RC-2-3T, were isolated from soil samples collected at Union Glacier, Antarctica. Based on 16S rRNA gene sequence similarity, strain EXRC-4A-4T was identified as belonging to the genus Rhodococcus, and strain RC-2-3T to the genus Pseudarthrobacter. Further genomic analyses, including average nucleotide identity and digital DNA-DNA hybridization, suggested that these strains represent new species. Strain EXRC-4A-4T exhibited growth at temperatures ranging from 4 to 28 °C (optimum between 20 and 28 °C), at pH 5.0-9.0 (optimum, pH 6.0), and in the presence of 0-5.0% NaCl (optimum between 0 and 1% NaCl). Strain RC-2-3T grew at 4-28 °C (optimum growth at 28 °C), pH 6.0-10 (optimum, pH 7.0) and in the presence of 0-5.0% NaCl (optimum, 1% NaCl). The fatty acid profile of EXRC-4A-4T was dominated by C17:1 ω-7, while that of RC-2-3T was dominated by anteiso-C15 : 0. The draft genome sequences revealed a DNA G+C content of 64.6 mol% for EXRC-4A-4T and 65.8 mol% for RC-2-3T. Based on this polyphasic study, EXRC-4A-4T and RC-2-3T represent two novel species within the genera Rhodococcus and Pseudarthrobacter, respectively. We propose the names Rhodococcus navarretei sp. nov. and Pseudarthrobacter quantipunctorum sp. nov. The type strains are Rhodococcus navarretei EXRC-4A-4T and Pseudarthrobacter quantipunctorum RC-2-3T. These strains have been deposited deposited in the CChRGM and BCCM/LMG culture collections with entry numbers RGM 3539/LMG 33621 and RGM 3538/LMG 33620, respectively.

Original languageEnglish
Article number006536
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume74
Issue number10
DOIs
Publication statusPublished - 1 Oct 2024

Keywords

  • Antarctica
  • Pseudarthrobacter
  • quantum dots
  • Rhodococcus
  • Union Glacier

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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