Removal of the endothelial layer in perfused mesenteric vascular bed of the rat

Teresa Pelissier, Hugo F. Miranda, Diego Bustamante, Carlos Paeile, Gianni Pinardi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The endothelial layer was removed from the isolated mesenteric vascular bed of the rat by perfusion with hypotonic Tyrode solution for 12.5 min. This procedure damaged more than 95% of the endothelial cells. After endothelial removal, the response to norepinephrine was significantly enhanced, whereas the relaxation induced by acetylcholine (ACh) was completely abolished. The results of this work show that perfusion with hypotonic solutions provides a reliable method of endothelial removal in isolated perfused vascular beds, allowing the study of endothelial-dependent vascular responses.

Original languageEnglish
Pages (from-to)41-44
Number of pages4
JournalJournal of Pharmacological and Toxicological Methods
Volume27
Issue number1
DOIs
Publication statusPublished - 1992

Keywords

  • Acetylcholine
  • Endothelium
  • Mesenteric vascular bed
  • Norepinephrine

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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