Removal of the endothelial layer in perfused mesenteric vascular bed of the rat

Teresa Pelissier; Hugo F. Miranda; Diego Bustamante; Carlos Paeile; Gianni Pinardi

doi:10.1016/1056-8719(92)90019-W

Removal of the endothelial layer in perfused mesenteric vascular bed of the rat

Teresa Pelissier, Hugo F. Miranda, Diego Bustamante, Carlos Paeile, Gianni Pinardi

Medicine School

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The endothelial layer was removed from the isolated mesenteric vascular bed of the rat by perfusion with hypotonic Tyrode solution for 12.5 min. This procedure damaged more than 95% of the endothelial cells. After endothelial removal, the response to norepinephrine was significantly enhanced, whereas the relaxation induced by acetylcholine (ACh) was completely abolished. The results of this work show that perfusion with hypotonic solutions provides a reliable method of endothelial removal in isolated perfused vascular beds, allowing the study of endothelial-dependent vascular responses.

Original language	English
Pages (from-to)	41-44
Number of pages	4
Journal	Journal of Pharmacological and Toxicological Methods
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/1056-8719(92)90019-W
Publication status	Published - 1992

Keywords

Acetylcholine
Endothelium
Mesenteric vascular bed
Norepinephrine

ASJC Scopus subject areas

Toxicology
Pharmacology

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10.1016/1056-8719(92)90019-W

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abstract = "The endothelial layer was removed from the isolated mesenteric vascular bed of the rat by perfusion with hypotonic Tyrode solution for 12.5 min. This procedure damaged more than 95% of the endothelial cells. After endothelial removal, the response to norepinephrine was significantly enhanced, whereas the relaxation induced by acetylcholine (ACh) was completely abolished. The results of this work show that perfusion with hypotonic solutions provides a reliable method of endothelial removal in isolated perfused vascular beds, allowing the study of endothelial-dependent vascular responses.",

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AU - Miranda, Hugo F.

AU - Bustamante, Diego

AU - Paeile, Carlos

AU - Pinardi, Gianni

PY - 1992

Y1 - 1992

N2 - The endothelial layer was removed from the isolated mesenteric vascular bed of the rat by perfusion with hypotonic Tyrode solution for 12.5 min. This procedure damaged more than 95% of the endothelial cells. After endothelial removal, the response to norepinephrine was significantly enhanced, whereas the relaxation induced by acetylcholine (ACh) was completely abolished. The results of this work show that perfusion with hypotonic solutions provides a reliable method of endothelial removal in isolated perfused vascular beds, allowing the study of endothelial-dependent vascular responses.

AB - The endothelial layer was removed from the isolated mesenteric vascular bed of the rat by perfusion with hypotonic Tyrode solution for 12.5 min. This procedure damaged more than 95% of the endothelial cells. After endothelial removal, the response to norepinephrine was significantly enhanced, whereas the relaxation induced by acetylcholine (ACh) was completely abolished. The results of this work show that perfusion with hypotonic solutions provides a reliable method of endothelial removal in isolated perfused vascular beds, allowing the study of endothelial-dependent vascular responses.

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KW - Endothelium

KW - Mesenteric vascular bed

KW - Norepinephrine

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ER -

Removal of the endothelial layer in perfused mesenteric vascular bed of the rat

Abstract

Keywords

ASJC Scopus subject areas

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