Recruitment and subnuclear distribution of the regulatory machinery during 1α,25-dihydroxy vitamin D3-mediated transcriptional upregulation in osteoblasts

Gloria Arriagada, Berta Henriquez, Daniel Moena, Paola Merino, Cinthya Ruiz-Tagle, Jane B. Lian, Gary S. Stein, Janet L. Stein, Martin Montecino

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The architectural organization of the genome and regulatory proteins within the nucleus supports gene expression in a physiologically regulated manner. In osteoblastic cells ligand activation induces a nuclear punctate distribution of the 1α,25-dihydroxy vitamin D3 (1α,25(OH)2D3) receptor (VDR) and promotes its interaction with transcriptional coactivators such as SRC-1, NCoA-62/Skip, and DRIP205. Here, we discuss evidence demonstrating that in osteoblastic cells VDR binds to the nuclear matrix fraction in a 1α,25(OH)2D3-dependent manner. This interaction occurs rapidly after exposure to 1α,25(OH)2D3 and does not require a functional VDR DNA binding domain. The nuclear matrix-bound VDR molecules colocalize with the also nuclear matrix-associated coactivator DRIP205. We propose a model where the rapid association of VDR with the nuclear matrix fraction represents an event that follows 1α,25(OH)2D3-dependent nuclear localization of VDR, but that precedes 1α,25(OH)2D3-dependent transcriptional upregulation at target genes.

Original languageEnglish
Pages (from-to)156-158
Number of pages3
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume121
Issue number1-2
DOIs
Publication statusPublished - Jul 2010

Keywords

  • Intranuclear trafficking
  • Nuclear matrix
  • Vitamin D receptor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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