Quantum dot-based assay for Cu2+ quantification in bacterial cell culture

V. Durán-Toro; A. Gran-Scheuch; N. Órdenes-Aenishanslins; J. P. Monrás; L. A. Saona; F. A. Venegas; T. G. Chasteen; D. Bravo; J. M. Pérez-Donoso

doi:10.1016/j.ab.2014.01.001

Quantum dot-based assay for Cu²⁺ quantification in bacterial cell culture

V. Durán-Toro, A. Gran-Scheuch, N. Órdenes-Aenishanslins, J. P. Monrás, L. A. Saona, F. A. Venegas, T. G. Chasteen, D. Bravo, J. M. Pérez-Donoso

Life Sciences School

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A simple and sensitive method for quantification of nanomolar copper with a detection limit of 1.2 × 10^-10 M and a linear range from 10^-9 to 10^-8 M is reported. For the most useful analytical concentration of quantum dots, 1160 μg/ml, a 1/K_sv value of 11 μM Cu²⁺ was determined. The method is based on the interaction of Cu²⁺ with glutathione-capped CdTe quantum dots (CdTe-GSH QDs) synthesized by a simple and economic biomimetic method. Green CdTe-GSH QDs displayed the best performance in copper quantification when QDs of different sizes/colors were tested. Cu²⁺ quantification is highly selective given that no significant interference of QDs with 19 ions was observed. No significant effects on Cu²⁺ quantification were determined when different reaction matrices such as distilled water, tap water, and different bacterial growth media were tested. The method was used to determine copper uptake kinetics on Escherichia coli cultures. QD-based quantification of copper on bacterial supernatants was compared with atomic absorption spectroscopy as a means of confirming the accuracy of the reported method. The mechanism of Cu²⁺-mediated QD fluorescence quenching was associated with nanoparticle decomposition.

Original language	English
Pages (from-to)	30-36
Number of pages	7
Journal	Analytical Biochemistry
Volume	450
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2014.01.001
Publication status	Published - 1 Apr 2014

Keywords

Bacterial copper uptake kinetics
Biomimetic synthesis
CdTe-GSH
Copper quantification
Quantum dots
Stern-Volmer

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2014.01.001

Cite this

@article{4ed5cf4d4bdd43b7b42c34b8606eab58,

title = "Quantum dot-based assay for Cu2+ quantification in bacterial cell culture",

abstract = "A simple and sensitive method for quantification of nanomolar copper with a detection limit of 1.2 × 10-10 M and a linear range from 10-9 to 10-8 M is reported. For the most useful analytical concentration of quantum dots, 1160 μg/ml, a 1/Ksv value of 11 μM Cu2+ was determined. The method is based on the interaction of Cu2+ with glutathione-capped CdTe quantum dots (CdTe-GSH QDs) synthesized by a simple and economic biomimetic method. Green CdTe-GSH QDs displayed the best performance in copper quantification when QDs of different sizes/colors were tested. Cu2+ quantification is highly selective given that no significant interference of QDs with 19 ions was observed. No significant effects on Cu2+ quantification were determined when different reaction matrices such as distilled water, tap water, and different bacterial growth media were tested. The method was used to determine copper uptake kinetics on Escherichia coli cultures. QD-based quantification of copper on bacterial supernatants was compared with atomic absorption spectroscopy as a means of confirming the accuracy of the reported method. The mechanism of Cu2+-mediated QD fluorescence quenching was associated with nanoparticle decomposition.",

keywords = "Bacterial copper uptake kinetics, Biomimetic synthesis, CdTe-GSH, Copper quantification, Quantum dots, Stern-Volmer",

author = "V. Dur{\'a}n-Toro and A. Gran-Scheuch and N. {\'O}rdenes-Aenishanslins and Monr{\'a}s, {J. P.} and Saona, {L. A.} and Venegas, {F. A.} and Chasteen, {T. G.} and D. Bravo and P{\'e}rez-Donoso, {J. M.}",

note = "Funding Information: This work was supported by Fondecyt 11110077 (J.M.P.), 11110076 (D.B.), Anillo ACT 1107 (J.M.P. and V.D.), Anillo ACT 1111 (D.B. and J.M.P.), and INACH grant T-19_11 (J.M.P. and D.B.). T.G.C. gratefully acknowledges support from the Robert A. Welch Foundation (X-011). A doctoral fellowship from CONICYT (Comisi{\'o}n Nacional de Ciencia y Tecnolog{\'i}a) to J.P.M. is also acknowledged. ",

year = "2014",

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doi = "10.1016/j.ab.2014.01.001",

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T1 - Quantum dot-based assay for Cu2+ quantification in bacterial cell culture

AU - Durán-Toro, V.

AU - Gran-Scheuch, A.

AU - Órdenes-Aenishanslins, N.

AU - Monrás, J. P.

AU - Saona, L. A.

AU - Venegas, F. A.

AU - Chasteen, T. G.

AU - Bravo, D.

AU - Pérez-Donoso, J. M.

N1 - Funding Information: This work was supported by Fondecyt 11110077 (J.M.P.), 11110076 (D.B.), Anillo ACT 1107 (J.M.P. and V.D.), Anillo ACT 1111 (D.B. and J.M.P.), and INACH grant T-19_11 (J.M.P. and D.B.). T.G.C. gratefully acknowledges support from the Robert A. Welch Foundation (X-011). A doctoral fellowship from CONICYT (Comisión Nacional de Ciencia y Tecnología) to J.P.M. is also acknowledged.

PY - 2014/4/1

Y1 - 2014/4/1

N2 - A simple and sensitive method for quantification of nanomolar copper with a detection limit of 1.2 × 10-10 M and a linear range from 10-9 to 10-8 M is reported. For the most useful analytical concentration of quantum dots, 1160 μg/ml, a 1/Ksv value of 11 μM Cu2+ was determined. The method is based on the interaction of Cu2+ with glutathione-capped CdTe quantum dots (CdTe-GSH QDs) synthesized by a simple and economic biomimetic method. Green CdTe-GSH QDs displayed the best performance in copper quantification when QDs of different sizes/colors were tested. Cu2+ quantification is highly selective given that no significant interference of QDs with 19 ions was observed. No significant effects on Cu2+ quantification were determined when different reaction matrices such as distilled water, tap water, and different bacterial growth media were tested. The method was used to determine copper uptake kinetics on Escherichia coli cultures. QD-based quantification of copper on bacterial supernatants was compared with atomic absorption spectroscopy as a means of confirming the accuracy of the reported method. The mechanism of Cu2+-mediated QD fluorescence quenching was associated with nanoparticle decomposition.

AB - A simple and sensitive method for quantification of nanomolar copper with a detection limit of 1.2 × 10-10 M and a linear range from 10-9 to 10-8 M is reported. For the most useful analytical concentration of quantum dots, 1160 μg/ml, a 1/Ksv value of 11 μM Cu2+ was determined. The method is based on the interaction of Cu2+ with glutathione-capped CdTe quantum dots (CdTe-GSH QDs) synthesized by a simple and economic biomimetic method. Green CdTe-GSH QDs displayed the best performance in copper quantification when QDs of different sizes/colors were tested. Cu2+ quantification is highly selective given that no significant interference of QDs with 19 ions was observed. No significant effects on Cu2+ quantification were determined when different reaction matrices such as distilled water, tap water, and different bacterial growth media were tested. The method was used to determine copper uptake kinetics on Escherichia coli cultures. QD-based quantification of copper on bacterial supernatants was compared with atomic absorption spectroscopy as a means of confirming the accuracy of the reported method. The mechanism of Cu2+-mediated QD fluorescence quenching was associated with nanoparticle decomposition.

KW - Bacterial copper uptake kinetics

KW - Biomimetic synthesis

KW - CdTe-GSH

KW - Copper quantification

KW - Quantum dots

KW - Stern-Volmer

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U2 - 10.1016/j.ab.2014.01.001

DO - 10.1016/j.ab.2014.01.001

M3 - Article

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SN - 0003-2697

VL - 450

SP - 30

EP - 36

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

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