Quantitation of RU 486 in human plasma by HPLC and RIA after column chromatography

Oskari Heikinheimo; Marjatta Tevilin; Donna Shoupe; Horacio Croxatto; Pekka Lähteenmäki

doi:10.1016/S0010-7824(86)80017-8

Quantitation of RU 486 in human plasma by HPLC and RIA after column chromatography

Oskari Heikinheimo, Marjatta Tevilin, Donna Shoupe, Horacio Croxatto, Pekka Lähteenmäki

Medicine School

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Chromosorb^R column chromatography was used for separation of RU 486 from its immunologically cross-reacting metabolites prior to quantitative analysis by radioimmunoassay (RIA) or high-performanceliquid chromatography (HPLC). The results of the two assay methods were in good agreement with each other (r=0.99, n=29). The retention time of RU 486 in our HPLC system was 2.5 min. Plasma concentrations of RU 486 were measured by HPLC up to 48 h following single oral administration of 100, 400, 600 and 800 mg of RU 486 to female volunteers. The plasma peak concentrations (2.0 - 2.5 μg/ml) were reached within the first hour. After redistribution, the plasma concentrations of RU 486 were not significantly affected by the doses studied but remained in the same range throughout the 48 hours. The plasma half-life between 24 and 48 hours was 27 hours or more. We conclude that HPLC is valuable in studies on the metabolism and pharmacokinetics of RU 486, but a less laborious RIA method after Chromosorb^R column chromatography is suitable and gives reliable results in large-scale clinical studies.

Original language	English
Pages (from-to)	613-624
Number of pages	12
Journal	Contraception
Volume	34
Issue number	6
DOIs	https://doi.org/10.1016/S0010-7824(86)80017-8
Publication status	Published - 1986

ASJC Scopus subject areas

General Medicine
Obstetrics and Gynaecology

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title = "Quantitation of RU 486 in human plasma by HPLC and RIA after column chromatography",

abstract = "ChromosorbR column chromatography was used for separation of RU 486 from its immunologically cross-reacting metabolites prior to quantitative analysis by radioimmunoassay (RIA) or high-performanceliquid chromatography (HPLC). The results of the two assay methods were in good agreement with each other (r=0.99, n=29). The retention time of RU 486 in our HPLC system was 2.5 min. Plasma concentrations of RU 486 were measured by HPLC up to 48 h following single oral administration of 100, 400, 600 and 800 mg of RU 486 to female volunteers. The plasma peak concentrations (2.0 - 2.5 μg/ml) were reached within the first hour. After redistribution, the plasma concentrations of RU 486 were not significantly affected by the doses studied but remained in the same range throughout the 48 hours. The plasma half-life between 24 and 48 hours was 27 hours or more. We conclude that HPLC is valuable in studies on the metabolism and pharmacokinetics of RU 486, but a less laborious RIA method after ChromosorbR column chromatography is suitable and gives reliable results in large-scale clinical studies.",

author = "Oskari Heikinheimo and Marjatta Tevilin and Donna Shoupe and Horacio Croxatto and Pekka L{\"a}hteenm{\"a}ki",

year = "1986",

doi = "10.1016/S0010-7824(86)80017-8",

language = "English",

volume = "34",

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T1 - Quantitation of RU 486 in human plasma by HPLC and RIA after column chromatography

AU - Heikinheimo, Oskari

AU - Tevilin, Marjatta

AU - Shoupe, Donna

AU - Croxatto, Horacio

AU - Lähteenmäki, Pekka

PY - 1986

Y1 - 1986

N2 - ChromosorbR column chromatography was used for separation of RU 486 from its immunologically cross-reacting metabolites prior to quantitative analysis by radioimmunoassay (RIA) or high-performanceliquid chromatography (HPLC). The results of the two assay methods were in good agreement with each other (r=0.99, n=29). The retention time of RU 486 in our HPLC system was 2.5 min. Plasma concentrations of RU 486 were measured by HPLC up to 48 h following single oral administration of 100, 400, 600 and 800 mg of RU 486 to female volunteers. The plasma peak concentrations (2.0 - 2.5 μg/ml) were reached within the first hour. After redistribution, the plasma concentrations of RU 486 were not significantly affected by the doses studied but remained in the same range throughout the 48 hours. The plasma half-life between 24 and 48 hours was 27 hours or more. We conclude that HPLC is valuable in studies on the metabolism and pharmacokinetics of RU 486, but a less laborious RIA method after ChromosorbR column chromatography is suitable and gives reliable results in large-scale clinical studies.

AB - ChromosorbR column chromatography was used for separation of RU 486 from its immunologically cross-reacting metabolites prior to quantitative analysis by radioimmunoassay (RIA) or high-performanceliquid chromatography (HPLC). The results of the two assay methods were in good agreement with each other (r=0.99, n=29). The retention time of RU 486 in our HPLC system was 2.5 min. Plasma concentrations of RU 486 were measured by HPLC up to 48 h following single oral administration of 100, 400, 600 and 800 mg of RU 486 to female volunteers. The plasma peak concentrations (2.0 - 2.5 μg/ml) were reached within the first hour. After redistribution, the plasma concentrations of RU 486 were not significantly affected by the doses studied but remained in the same range throughout the 48 hours. The plasma half-life between 24 and 48 hours was 27 hours or more. We conclude that HPLC is valuable in studies on the metabolism and pharmacokinetics of RU 486, but a less laborious RIA method after ChromosorbR column chromatography is suitable and gives reliable results in large-scale clinical studies.

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U2 - 10.1016/S0010-7824(86)80017-8

DO - 10.1016/S0010-7824(86)80017-8

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C2 - 3829676

AN - SCOPUS:0022896969

SN - 0010-7824

VL - 34

SP - 613

EP - 624

JO - Contraception

JF - Contraception

IS - 6

ER -

Quantitation of RU 486 in human plasma by HPLC and RIA after column chromatography

Abstract

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