Purification and characterization of a human factor that assembles and remodels chromatin

Gary LeRoy, Alejandra Loyola, William S. Lane, Danny Reinberg

Research output: Contribution to journalArticlepeer-review

137 Citations (Scopus)

Abstract

We have previously reported the isolation and characterization of a nucleosome remodeling and spacing factor, RSF. One of the RSF subunits is hSNF2h, a SNF2 homologue. Here we set out to isolate and characterize other hSNF2h-containing complexes. We have identified a novel hSNF2h complex that facilitates ATP-dependent chromatin assembly with the histone chaperone NAP- 1. The complex possesses ATPase activity that is DNA-dependent and nucleosome-stimulated. This complex is capable of facilitating ATP-dependent nucleosome remodeling and transcription initiation from chromatin templates. In addition to hSNF2h, this complex also contains a 190-kDa protein encoded by the BAZ1A gene. Since both subunits are homologues of the Drosophila ACF complex (ATP-utilizing chromatin assembly and remodeling factor), we have named this factor human ACF or hACF.

Original languageEnglish
Pages (from-to)14787-14790
Number of pages4
JournalJournal of Biological Chemistry
Volume275
Issue number20
DOIs
Publication statusPublished - 19 May 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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