Proline-rich extensin-like receptor kinases PERK5 and PERK12 are involved in pollen tube growth

Cecilia Borassi; Ana R. Sede; Martín A. Mecchia; Silvina Mangano; Eliana Marzol; Silvina P. Denita-Juarez; Juan D. Salgado Salter; Silvia M. Velasquez; Jorge P. Muschietti; José M. Estevez

doi:10.1002/1873-3468.14185

Proline-rich extensin-like receptor kinases PERK5 and PERK12 are involved in pollen tube growth

Cecilia Borassi, Ana R. Sede, Martín A. Mecchia, Silvina Mangano, Eliana Marzol, Silvina P. Denita-Juarez, Juan D. Salgado Salter, Silvia M. Velasquez, Jorge P. Muschietti, José M. Estevez

Universidad Andrés Bello

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Proline-rich extensin-like receptor kinases (PERKs) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily known to be involved in many plant developmental processes. Here, we characterized two pollen-expressed PERKs from Arabidopsis thaliana, PERK5 and PERK12. Pollen tube growth was impaired in single and double perk5-1 perk12-1 loss of function mutants, with an impact on seed production. When the segregation was analysed, a male gametophytic defect was found, indicating that perk5-1 and perk12-1 mutants carry deficient pollen transmission. Furthermore, perk5-1 perk12-1 displayed an excessive accumulation of pectins and cellulose at the cell wall of the pollen tubes. Our results indicate that PERK5 and PERK12 are necessary for proper pollen tube growth, highlighting their role in cell wall assembly and reactive oxygen species homeostasis.

Original language	English
Pages (from-to)	2593-2607
Number of pages	15
Journal	FEBS Letters
Volume	595
Issue number	20
DOIs	https://doi.org/10.1002/1873-3468.14185
Publication status	Accepted/In press - 2021

Keywords

Arabidopsis thaliana
cell wall
PERKs
polar growth
pollen tubes

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1002/1873-3468.14185

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@article{86dc985858eb4c66a2716dfaa9148703,

title = "Proline-rich extensin-like receptor kinases PERK5 and PERK12 are involved in pollen tube growth",

abstract = "Proline-rich extensin-like receptor kinases (PERKs) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily known to be involved in many plant developmental processes. Here, we characterized two pollen-expressed PERKs from Arabidopsis thaliana, PERK5 and PERK12. Pollen tube growth was impaired in single and double perk5-1 perk12-1 loss of function mutants, with an impact on seed production. When the segregation was analysed, a male gametophytic defect was found, indicating that perk5-1 and perk12-1 mutants carry deficient pollen transmission. Furthermore, perk5-1 perk12-1 displayed an excessive accumulation of pectins and cellulose at the cell wall of the pollen tubes. Our results indicate that PERK5 and PERK12 are necessary for proper pollen tube growth, highlighting their role in cell wall assembly and reactive oxygen species homeostasis.",

keywords = "Arabidopsis thaliana, cell wall, PERKs, polar growth, pollen tubes",

author = "Cecilia Borassi and Sede, {Ana R.} and Mecchia, {Mart{\'i}n A.} and Silvina Mangano and Eliana Marzol and Denita-Juarez, {Silvina P.} and {Salgado Salter}, {Juan D.} and Velasquez, {Silvia M.} and Muschietti, {Jorge P.} and Estevez, {Jos{\'e} M.}",

note = "Publisher Copyright: {\textcopyright} 2021 Federation of European Biochemical Societies",

year = "2021",

doi = "10.1002/1873-3468.14185",

language = "English",

volume = "595",

pages = "2593--2607",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "20",

}

TY - JOUR

T1 - Proline-rich extensin-like receptor kinases PERK5 and PERK12 are involved in pollen tube growth

AU - Borassi, Cecilia

AU - Sede, Ana R.

AU - Mecchia, Martín A.

AU - Mangano, Silvina

AU - Marzol, Eliana

AU - Denita-Juarez, Silvina P.

AU - Salgado Salter, Juan D.

AU - Velasquez, Silvia M.

AU - Muschietti, Jorge P.

AU - Estevez, José M.

PY - 2021

Y1 - 2021

N2 - Proline-rich extensin-like receptor kinases (PERKs) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily known to be involved in many plant developmental processes. Here, we characterized two pollen-expressed PERKs from Arabidopsis thaliana, PERK5 and PERK12. Pollen tube growth was impaired in single and double perk5-1 perk12-1 loss of function mutants, with an impact on seed production. When the segregation was analysed, a male gametophytic defect was found, indicating that perk5-1 and perk12-1 mutants carry deficient pollen transmission. Furthermore, perk5-1 perk12-1 displayed an excessive accumulation of pectins and cellulose at the cell wall of the pollen tubes. Our results indicate that PERK5 and PERK12 are necessary for proper pollen tube growth, highlighting their role in cell wall assembly and reactive oxygen species homeostasis.

AB - Proline-rich extensin-like receptor kinases (PERKs) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily known to be involved in many plant developmental processes. Here, we characterized two pollen-expressed PERKs from Arabidopsis thaliana, PERK5 and PERK12. Pollen tube growth was impaired in single and double perk5-1 perk12-1 loss of function mutants, with an impact on seed production. When the segregation was analysed, a male gametophytic defect was found, indicating that perk5-1 and perk12-1 mutants carry deficient pollen transmission. Furthermore, perk5-1 perk12-1 displayed an excessive accumulation of pectins and cellulose at the cell wall of the pollen tubes. Our results indicate that PERK5 and PERK12 are necessary for proper pollen tube growth, highlighting their role in cell wall assembly and reactive oxygen species homeostasis.

KW - Arabidopsis thaliana

KW - cell wall

KW - PERKs

KW - polar growth

KW - pollen tubes

UR - http://www.scopus.com/inward/record.url?scp=85114496446&partnerID=8YFLogxK

U2 - 10.1002/1873-3468.14185

DO - 10.1002/1873-3468.14185

M3 - Article

AN - SCOPUS:85114496446

SN - 0014-5793

VL - 595

SP - 2593

EP - 2607

JO - FEBS Letters

JF - FEBS Letters

IS - 20

ER -

Proline-rich extensin-like receptor kinases PERK5 and PERK12 are involved in pollen tube growth

Abstract

Keywords

ASJC Scopus subject areas

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