Abstract
The effect of seasonal acclimatization on the extent of prolactin (PRL) gene expression and on the content of this was studied in summer- and winter-carp (Cyprinus carpio) hormone pituitary glands. PRL content in the rostral pars distalis (RPD) was evaluated by immunocytochemistry using antibodies against a cross-linked synthetic peptide comprising the sequence of 15 amino acids which conform to the primary structure of carp PRL. To assess the level of PRL gene transcription, a 24-mer synthetic oligonucleotide probe whose sequence included nucleotides 2041-2064 located in exon V of the carp PRL gene, was used. Employing in situ hybridization assays, a high expression of PRL mRNA was observed in the RPD of summer-acclimatized carp. A negligible level of transcription was observed in tissue sections of pituitary glands from winter-acclimatized carp. Concurrently, immunodetection of the PRL-producing cells in the RPD revealed that the pituitary hormone level was significantly higher in the warm season-adapted carp.
Original language | English |
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Pages (from-to) | 551-560 |
Number of pages | 10 |
Journal | Comparative Biochemistry and Physiology -- Part B: Biochemistry and |
Volume | 108 |
Issue number | 4 |
DOIs | |
Publication status | Published - Aug 1994 |
Keywords
- Carp
- Cyprinus carpio
- Gene expression
- Pituitary
- Prolactin
- Seasonal acclimatization
- Teleost fish
- Temperature
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Molecular Biology