Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp

Jaime Figueroa, Alfredo Molina, Marco Alvarez, Julieta Villanueva, Ariel Reyes, Gloria León, Manuel Krauskopf

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

The effect of seasonal acclimatization on the extent of prolactin (PRL) gene expression and on the content of this was studied in summer- and winter-carp (Cyprinus carpio) hormone pituitary glands. PRL content in the rostral pars distalis (RPD) was evaluated by immunocytochemistry using antibodies against a cross-linked synthetic peptide comprising the sequence of 15 amino acids which conform to the primary structure of carp PRL. To assess the level of PRL gene transcription, a 24-mer synthetic oligonucleotide probe whose sequence included nucleotides 2041-2064 located in exon V of the carp PRL gene, was used. Employing in situ hybridization assays, a high expression of PRL mRNA was observed in the RPD of summer-acclimatized carp. A negligible level of transcription was observed in tissue sections of pituitary glands from winter-acclimatized carp. Concurrently, immunodetection of the PRL-producing cells in the RPD revealed that the pituitary hormone level was significantly higher in the warm season-adapted carp.

Original languageEnglish
Pages (from-to)551-560
Number of pages10
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume108
Issue number4
DOIs
Publication statusPublished - Aug 1994

Keywords

  • Carp
  • Cyprinus carpio
  • Gene expression
  • Pituitary
  • Prolactin
  • Seasonal acclimatization
  • Teleost fish
  • Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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