Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis

Vivian Wilhelm, Cristián Soza, Rodgrigo Martínez, Mario Rosemblatt, Luis O. Burzio, Pablo D T Valenzuela

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.

Original languageEnglish
Pages (from-to)69-82
Number of pages14
JournalBiological Research
Volume38
Issue number1
DOIs
Publication statusPublished - 2005

Keywords

  • Hsp60
  • Hsp70
  • Immune response
  • Piscirickettsia salmonis
  • dnaK
  • groEL

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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