TY - JOUR
T1 - Primary structure and transcription of an amplified genetic locus
T2 - The CUP1 locus of yeast
AU - Karin, M.
AU - Najarian, R.
AU - Haslinger, A.
AU - Valenzuela, P.
AU - Welch, J.
AU - Fogel, S.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1984
Y1 - 1984
N2 - Copper resistance in yeast is controlled by the CUP1 locus. The level of resistance is proportional to the copy number of this locus, which can be found in up to 15 tandemly iterated copies. To elucidate the molecular mechanisms controlling the amplification and expression of the CUP1 locus, we determined its full nucleotide sequence. We have also identified and mapped two transcription units within the basic amplification unit of CUP1 in laboratory yeast strains. One of those transcription units is inducible by copper and encodes a low molecular weight copper binding protein-copper chelatin. The increased production of chelatin, due to both gene amplification and induction of transcription, leads to increased resistance of yeast cell to copper ions.
AB - Copper resistance in yeast is controlled by the CUP1 locus. The level of resistance is proportional to the copy number of this locus, which can be found in up to 15 tandemly iterated copies. To elucidate the molecular mechanisms controlling the amplification and expression of the CUP1 locus, we determined its full nucleotide sequence. We have also identified and mapped two transcription units within the basic amplification unit of CUP1 in laboratory yeast strains. One of those transcription units is inducible by copper and encodes a low molecular weight copper binding protein-copper chelatin. The increased production of chelatin, due to both gene amplification and induction of transcription, leads to increased resistance of yeast cell to copper ions.
UR - http://www.scopus.com/inward/record.url?scp=2042423392&partnerID=8YFLogxK
U2 - 10.1073/pnas.81.2.337
DO - 10.1073/pnas.81.2.337
M3 - Article
C2 - 6364141
AN - SCOPUS:2042423392
VL - 81
SP - 337
EP - 341
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 2 I
ER -