Poly

Luis Burzio, Lillian Reich, S. S. Koide

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Poly(adenosine diphosphori-bose) (ADPR) synthase activities of nuclei isolated from normal human and leukemic leukocytes were assayed by incubation with radioactive NAD+. The synthase activity of leukemic leukocyte nuclei was significantly higher than that of normal leukocyte nuclei. The average length of polymers formed by isolated leukemic nuclei under the prescribed experimental condition ranged from 3.1 to 5.3 ADPR residues per chain, while those produced by normal leukocytes nuclei was 1.7 and 2.6 residues per chain. Isolated leukemic and normal leukocyte nuclei were incubated with and without NAD+ and the ability to carry out DNA synthesis was measured. The endogenous DNA synthesis of NAD-treated and untreated nuclei was the same. This finding parallels the result obtained with Novikoff hepatoma cell nuclei and differs from the observation with rat liver or testis nuclei. The authors are grateful to Mrs. M. Elvira for the technical assistance. The study was supported in part by USPHS Grant P01 HD05671, from The National Institute of Child Health and Human Development.

Original languageEnglish
Pages (from-to)933-938
Number of pages6
JournalExperimental Biology and Medicine
Volume149
Issue number4
DOIs
Publication statusPublished - 8 Sep 1975
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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