Polarized trafficking of thyrocyte proteins in MDCK cells

Xiaoqing Zhang, Claudia Riedel, Nancy Carrasco, Peter Arvan

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Recent studies suggest striking similarities between polarized protein sorting in thyrocytes and MDCK epithelial cells, including apical trafficking of thyroglobulin (Tg), thyroid peroxidase, and aminopeptidase N; as well as basolateral targeting of heparan sulfate proteoglycans, thrombospondin 1 (TSP1), type 1 5'-deiodinase, sodium-potassium ATPase, and the thyrotropin receptor. In this report, we have firstly expressed in stably transfected MDCK II cells a range of truncation mutants lacking up to 78% of the C-terminus of TSP1; these studies indicate that the N-terminal region containing the heparin binding domain is sufficient for basolateral targeting of TSP1. Secondly, we have stably transfected MDCK II cells with both Tg and sodium-iodide symporter (NIS) cDNAs, obtaining clones that simultaneously express both thyroid-specific proteins at the apical and basolateral cell surfaces, respectively. These studies represent promising early steps towards designing artificial thyrocytes by thyroid gene transfer into MDCK cells.

Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume188
Issue number1-2
DOIs
Publication statusPublished - 25 Feb 2002

Keywords

  • Biotinylation
  • Epithelial cells
  • Trafficking

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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