Plasma membrane destination of the classical Xenopus laevis progesterone receptor accelerates progesterone-induced oocyte maturation

Silvana Martinez, Rodrigo Grandy, Pamela Pasten, Hernán Montecinos, Martín Montecino, Juan Olate, María Victoria Hinrichs

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Xenopus laevis oocyte maturation is induced by the steroid hormone progesterone through a non-genomic mechanism initiated at the cell membrane. Recently, two Xenopus oocyte progesterone receptors have been cloned; one is the classical progesterone receptor (xPR-1) involved in genomic actions and the other a putative seven-transmembrane-G-protein-couple receptor. Both receptors are postulated to be mediating the steroid-induced maturation process in the frog oocyte. In this study, we tested the hypothesis that the classical progesterone receptor, associated to the oocyte plasma membrane, is participating in the reinitiation of the cell cycle. Addition of a myristoilation and palmytoilation signal at the amino terminus of xPR-1 (mp xPR-1), increased the amount of receptor associated to the oocyte plasma membrane and most importantly, significantly potentiated progesterone-induced oocyte maturation sensitivity. These findings suggest that the classical xPR-1, located at the plasma membrane, is mediating through a non-genomic mechanism, the reinitiation of the meiotic cell cycle in the X. laevis oocyte.

Original languageEnglish
Pages (from-to)853-859
Number of pages7
JournalJournal of Cellular Biochemistry
Volume99
Issue number3
DOIs
Publication statusPublished - 15 Oct 2006

Keywords

  • Progesterone
  • Progesterone receptor
  • Xenopus oocyte

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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