TY - JOUR
T1 - Physicochemical assessment of Dextran-g-Poly (ε-caprolactone) micellar nanoaggregates as drug nanocarriers
AU - Saldías, César
AU - Velásquez, Luis
AU - Quezada, Caterina
AU - Leiva, Angel
N1 - Funding Information:
The authors thank the Fondecyt Grants 1120712 and 1120119 and R08C1002 project (CIPA, GORE BIO BIO, Conicyt Regional) for partial financial support of this research.
Publisher Copyright:
© 2014 Elsevier Ltd. All rights reserved.
PY - 2015/3/6
Y1 - 2015/3/6
N2 - Self-assembling polymers in aqueous solution have attracted significant attention with recent research efforts focused on the development of new strategies to design devices useful in the field of controlled drug delivery. In this context, amphiphilic copolymers having specific structural features and self-assembling behaviors in aqueous media that would enable controlled drug release over longer time periods. In this work, we report on the synthesis and characterization of a Poly (ε-caprolactone)-grafted Dextran copolymer and its use in the preparation of micellar nanoaggregates. The characterization and study of the morphology, topography, size distribution and stability of micellar nanoaggregates by Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), Dynamic Light Scattering (DLS) and Zeta Potential (ζ), respectively, were carried out. Spherical-shaped morphologies and an average size of approximately 83 nm, for drug-free nanoaggregates, were observed. In addition, Zeta Potential studies showed that drug-free nanoaggregates are more stable than drug-loaded structures measured in a phosphate buffer (pH 7.2) medium. UV-vis spectrophotometry of both the drug entrapment efficiency (EE%) and in vitro drug release behavior were assessed. The EE% was determined to be 78% (w/w), and a combination of diffusion and eroding polymer matrix mechanisms for drug release were established. Finally, these results indicate that Dx-g-PCL micellar nanoaggregates are suitable for use as a potential nanocarrier having both biodegradable and biocompatible properties.
AB - Self-assembling polymers in aqueous solution have attracted significant attention with recent research efforts focused on the development of new strategies to design devices useful in the field of controlled drug delivery. In this context, amphiphilic copolymers having specific structural features and self-assembling behaviors in aqueous media that would enable controlled drug release over longer time periods. In this work, we report on the synthesis and characterization of a Poly (ε-caprolactone)-grafted Dextran copolymer and its use in the preparation of micellar nanoaggregates. The characterization and study of the morphology, topography, size distribution and stability of micellar nanoaggregates by Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), Dynamic Light Scattering (DLS) and Zeta Potential (ζ), respectively, were carried out. Spherical-shaped morphologies and an average size of approximately 83 nm, for drug-free nanoaggregates, were observed. In addition, Zeta Potential studies showed that drug-free nanoaggregates are more stable than drug-loaded structures measured in a phosphate buffer (pH 7.2) medium. UV-vis spectrophotometry of both the drug entrapment efficiency (EE%) and in vitro drug release behavior were assessed. The EE% was determined to be 78% (w/w), and a combination of diffusion and eroding polymer matrix mechanisms for drug release were established. Finally, these results indicate that Dx-g-PCL micellar nanoaggregates are suitable for use as a potential nanocarrier having both biodegradable and biocompatible properties.
KW - Dextran
KW - Drug delivery
KW - PCL
KW - Polymeric nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84908406137&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2014.09.035
DO - 10.1016/j.carbpol.2014.09.035
M3 - Article
C2 - 25498659
AN - SCOPUS:84908406137
SN - 0144-8617
VL - 117
SP - 458
EP - 467
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -