TY - JOUR
T1 - Persistent Salmonella enterica serovar Typhimurium infection increases the susceptibility of mice to develop intestinal inflammation
AU - Schultz, Bárbara M.
AU - Salazar, Geraldyne A.
AU - Paduro, Carolina A.
AU - Pardo-Roa, Catalina
AU - Pizarro, Daniela P.
AU - Salazar-Echegarai, Francisco J.
AU - Torres, Javiera
AU - Riedel, Claudia A.
AU - Kalergis, Alexis M.
AU - álvarez-Lobos, Manuel M.
AU - Bueno, Susan M.
N1 - Funding Information:
This study was supported by grants 'Fondo Nacional de Ciencia y Tecnolog?a de Chile' (FONDECYT) (# 1100971, 1110604, 1140010, 1150862, 1161525, 1131012, and 1170964); Millenium Institute on Immunology and Immunotherapy P09/016-F from the Iniciativa Cient?fica Milenio, Ministry of Economy, Chilean Government; Proyecto Puente P 1715/2017, Direcci?n de Investigaci?n, Vicerrector?a de Investigaci?n, Pontificia Universidad Cat?lica de Chile. BS and CP-R are supported by the Comisi?n Nacional de Investigaci?n Cient?fica y Tecnol?gica (CONICYT).
Publisher Copyright:
© 2018 Schultz, Salazar, Paduro, Pardo-Roa, Pizarro, Salazar-Echegarai, Torres, Riedel, Kalergis, álvarez-Lobos and Bueno.
PY - 2018/5/29
Y1 - 2018/5/29
N2 - Chronic intestinal inflammations are triggered by genetic and environmental components. However, it remains unclear how specific changes in the microbiota, host immunity, or pathogen exposure could promote the onset and exacerbation of these diseases. Here, we evaluated whether Salmonella enterica serovar Typhimurium (S. Typhimurium) infection increases the susceptibility to develop intestinal inflammation in mice. Two mouse models were used to evaluate the impact of S. Typhimurium infection: the chemical induction of colitis by dextran sulfate sodium (DSS) and interleukin (IL)-10-/- mice, which develop spontaneous intestinal inflammation. We observed that S. Typhimurium infection makes DSS-treated and IL-10-/- mice more susceptible to develop intestinal inflammation. Importantly, this increased susceptibility is associated to the ability of S. Typhimurium to persist in liver and spleen of infected mice, which depends on the virulence proteins secreted by Salmonella Pathogenicity Island 2-encoded type three secretion system (TTSS-2). Although immunization with a live attenuated vaccine resulted in a moderate reduction of the IL-10-/- mice susceptibility to develop intestinal inflammation due to previous S. Typhimurium infection, it did not prevent bacterial persistence. Our results suggest that persistent S. Typhimurium infection may increase the susceptibility of mice to develop inflammation in the intestine, which could be associated with virulence proteins secreted by TTSS-2.
AB - Chronic intestinal inflammations are triggered by genetic and environmental components. However, it remains unclear how specific changes in the microbiota, host immunity, or pathogen exposure could promote the onset and exacerbation of these diseases. Here, we evaluated whether Salmonella enterica serovar Typhimurium (S. Typhimurium) infection increases the susceptibility to develop intestinal inflammation in mice. Two mouse models were used to evaluate the impact of S. Typhimurium infection: the chemical induction of colitis by dextran sulfate sodium (DSS) and interleukin (IL)-10-/- mice, which develop spontaneous intestinal inflammation. We observed that S. Typhimurium infection makes DSS-treated and IL-10-/- mice more susceptible to develop intestinal inflammation. Importantly, this increased susceptibility is associated to the ability of S. Typhimurium to persist in liver and spleen of infected mice, which depends on the virulence proteins secreted by Salmonella Pathogenicity Island 2-encoded type three secretion system (TTSS-2). Although immunization with a live attenuated vaccine resulted in a moderate reduction of the IL-10-/- mice susceptibility to develop intestinal inflammation due to previous S. Typhimurium infection, it did not prevent bacterial persistence. Our results suggest that persistent S. Typhimurium infection may increase the susceptibility of mice to develop inflammation in the intestine, which could be associated with virulence proteins secreted by TTSS-2.
KW - Colitis
KW - Dextran sulfate sodium
KW - Inflammatory bowel disease
KW - Interleukin-10
KW - Persistence
KW - Salmonella enterica serovar Typhimurium
UR - http://www.scopus.com/inward/record.url?scp=85047662061&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2018.01166
DO - 10.3389/fimmu.2018.01166
M3 - Article
AN - SCOPUS:85047662061
SN - 1664-3224
VL - 9
JO - Frontiers in Immunology
JF - Frontiers in Immunology
IS - MAY
M1 - 1166
ER -