Pectin Methylesterases Modulate Plant Homogalacturonan Status in Defenses against the Aphid Myzus persicae

Christian Silva-Sanzana, Jonathan Celiz-Balboa, Elisa Garzo, Susan E. Marcus, Juan Pablo Parra-Rojas, Barbara Rojas, Patricio Olmedo, Miguel A. Rubilar, Ignacio Rios, Rodrigo A. Chorbadjian, Alberto Fereres, Paul Knox, Susana Saez-Aguayo, Francisca Blanco-Herrera

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Abstract

Because they suck phloem sap and act as vectors for phytopathogenic viruses, aphids pose a threat to crop yields worldwide. Pectic homogalacturonan (HG) has been described as a defensive element for plants during infections with phytopathogens. However, its role during aphid infestation remains unexplored. Using immunofluorescence assays and biochemical approaches, the HG methylesterification status and associated modifying enzymes during the early stage of Arabidopsis (Arabidopsis thaliana) infestation with the green peach aphid (Myzus persicae) were analyzed. Additionally, the influence of pectin methylesterase (PME) activity on aphid settling and feeding behavior was evaluated by free choice assays and the Electrical Penetration Graph technique, respectively. Our results revealed that HG status and HG-modifying enzymes are significantly altered during the early stage of the plant-aphid interaction. Aphid infestation induced a significant increase in total PME activity and methanol emissions, concomitant with a decrease in the degree of HG methylesterification. Conversely, inhibition of PME activity led to a significant decrease in the settling and feeding preference of aphids. Furthermore, we demonstrate that the PME inhibitor AtPMEI13 has a defensive role during aphid infestation, since pmei13 mutants are significantly more susceptible to M. persicae in terms of settling preference, phloem access, and phloem sap drainage.

Original languageEnglish
Pages (from-to)1913-1929
Number of pages17
JournalPlant Cell
Volume31
Issue number8
DOIs
Publication statusPublished - 1 Aug 2019

ASJC Scopus subject areas

  • Cell Biology
  • Plant Science

Cite this

Silva-Sanzana, C., Celiz-Balboa, J., Garzo, E., Marcus, S. E., Pablo Parra-Rojas, J., Rojas, B., Olmedo, P., Rubilar, M. A., Rios, I., Chorbadjian, R. A., Fereres, A., Knox, P., Saez-Aguayo, S., & Blanco-Herrera, F. (2019). Pectin Methylesterases Modulate Plant Homogalacturonan Status in Defenses against the Aphid Myzus persicae. Plant Cell, 31(8), 1913-1929. https://doi.org/10.1105/tpc.19.00136