TY - JOUR
T1 - Pathogen typing in the genomics era
T2 - MLST and the future of molecular epidemiology
AU - Pérez-Losada, Marcos
AU - Cabezas, Patricia
AU - Castro-Nallar, Eduardo
AU - Crandall, Keith A.
N1 - Funding Information:
We thank Michel Tibayrenc for inviting us to write this review. We were supported by CIBIO (Portugal) and George Washington University. P.C. was funded by a fellowship from Fundación Caja Madrid, Spain . E.C.N. was funded by Comisión Nacional de Investigación Científica y Tecnológica (CONICYT), Gobierno de Chile, Becas , and by BYU Graduate Mentoring (2011) and Research (2012) Awards .
PY - 2013/6
Y1 - 2013/6
N2 - Multi-locus sequence typing (MLST) is a high-resolution genetic typing approach to identify species and strains of pathogens impacting human health, agriculture (animals and plants), and biosafety. In this review, we outline the general concepts behind MLST, molecular approaches for obtaining MLST data, analytical approaches for MLST data, and the contributions MLST studies have made in a wide variety of areas. We then look at the future of MLST and their relative strengths and weaknesses with respect to whole genome sequence typing approaches that are moving into the research arena at an ever-increasing pace. Throughout the paper, we provide exemplar references of these various aspects of MLST. The literature is simply too vast to make this review comprehensive, nevertheless, we have attempted to include enough references in a variety of key areas to introduce the reader to the broad applications and complications of MLST data.
AB - Multi-locus sequence typing (MLST) is a high-resolution genetic typing approach to identify species and strains of pathogens impacting human health, agriculture (animals and plants), and biosafety. In this review, we outline the general concepts behind MLST, molecular approaches for obtaining MLST data, analytical approaches for MLST data, and the contributions MLST studies have made in a wide variety of areas. We then look at the future of MLST and their relative strengths and weaknesses with respect to whole genome sequence typing approaches that are moving into the research arena at an ever-increasing pace. Throughout the paper, we provide exemplar references of these various aspects of MLST. The literature is simply too vast to make this review comprehensive, nevertheless, we have attempted to include enough references in a variety of key areas to introduce the reader to the broad applications and complications of MLST data.
KW - Molecular epidemiology
KW - Multi-locus sequence typing (MLST)
KW - Next-generation sequence data
KW - Pathogen evolution
UR - http://www.scopus.com/inward/record.url?scp=84875255868&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2013.01.009
DO - 10.1016/j.meegid.2013.01.009
M3 - Review article
C2 - 23357583
AN - SCOPUS:84875255868
SN - 1567-1348
VL - 16
SP - 38
EP - 53
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -
