Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi; Juliana Pérez Di Giorgio; María R. Scarpin; Jorge Muschietti; José M. Estevez

doi:10.1007/978-1-4939-1902-4_3

Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi, Juliana Pérez Di Giorgio, María R. Scarpin, Jorge Muschietti, José M. Estevez

Life Sciences School

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

Original language	English
Title of host publication	Plant Cell Expansion
Subtitle of host publication	Methods and Protocols
Publisher	Springer New York LLC
Pages	41-47
Number of pages	7
ISBN (Electronic)	9781493919024
ISBN (Print)	9781493919017
DOIs	https://doi.org/10.1007/978-1-4939-1902-4_3
Publication status	Published - 18 Nov 2014

Keywords

Arabidopsis pollen
Cell expansion
Pollen tube growth

ASJC Scopus subject areas

General Agricultural and Biological Sciences
General Medicine

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10.1007/978-1-4939-1902-4_3

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abstract = "Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.",

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AU - Borassi, Cecilia

AU - Di Giorgio, Juliana Pérez

AU - Scarpin, María R.

AU - Muschietti, Jorge

AU - Estevez, José M.

PY - 2014/11/18

Y1 - 2014/11/18

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AB - Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

KW - Arabidopsis pollen

KW - Cell expansion

KW - Pollen tube growth

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ER -

Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Abstract

Keywords

ASJC Scopus subject areas

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