Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi, Juliana Pérez Di Giorgio, María R. Scarpin, Jorge Muschietti, José M. Estevez

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)


Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

Original languageEnglish
Title of host publicationPlant Cell Expansion
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York LLC
Number of pages7
ISBN (Electronic)9781493919024
ISBN (Print)9781493919017
Publication statusPublished - 18 Nov 2014


  • Arabidopsis pollen
  • Cell expansion
  • Pollen tube growth

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • General Medicine


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