Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi, Juliana Pérez Di Giorgio, María R. Scarpin, Jorge Muschietti, José M. Estevez

Research output: Contribution to journalArticle

Abstract

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalMethods in Molecular Biology
Volume1242
DOIs
Publication statusPublished - 1 Jan 2015

Fingerprint

Pollen Tube
Arabidopsis
Growth
Ovule
Cell Polarity
Insertional Mutagenesis
Fertilization
Spermatozoa
In Vitro Techniques
Reactive Oxygen Species
Ions
Calcium
Phenotype

Keywords

  • Arabidopsis pollen
  • Cell expansion
  • Pollen tube growth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Borassi, Cecilia ; Di Giorgio, Juliana Pérez ; Scarpin, María R. ; Muschietti, Jorge ; Estevez, José M. / Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. In: Methods in Molecular Biology. 2015 ; Vol. 1242. pp. 41-47.
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Optimized method for growing in vitro Arabidopsis thaliana pollen tubes. / Borassi, Cecilia; Di Giorgio, Juliana Pérez; Scarpin, María R.; Muschietti, Jorge; Estevez, José M.

In: Methods in Molecular Biology, Vol. 1242, 01.01.2015, p. 41-47.

Research output: Contribution to journalArticle

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