Optimized method for growing in vitro Arabidopsis thaliana pollen tubes

Cecilia Borassi, Juliana Pérez Di Giorgio, María R. Scarpin, Jorge Muschietti, José M. Estevez

Research output: Contribution to journalArticlepeer-review

Abstract

Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalMethods in Molecular Biology
Volume1242
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Arabidopsis pollen
  • Cell expansion
  • Pollen tube growth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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