Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish

Juan P. García-López, Alexandre Grimaldi, Zelin Chen, Claudio Meneses, Karina Bravo-Tello, Erica Bresciani, Alvaro Banderas, Shawn M. Burgess, Pedro P. Hernández, Carmen G. Feijoo

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The current view of hematopoiesis considers leukocytes on a continuum with distinct developmental origins, and which exert non-overlapping functions. However, there is less known about the function and phenotype of ontogenetically distinct neutrophil populations. In this work, using a photoconvertible transgenic zebrafish line; Tg(mpx:Dendra2), we selectively label rostral blood island-derived and caudal hematopoietic tissue-derived neutrophils in vivo during steady state or upon injury. By comparing the migratory properties and single-cell expression profiles of both neutrophil populations at steady state we show that rostral neutrophils show higher csf3b expression and migration capacity than caudal neutrophils. Upon injury, both populations share a core transcriptional profile as well as subset-specific transcriptional signatures. Accordingly, both rostral and caudal neutrophils are recruited to the wound independently of their distance to the injury. While rostral neutrophils respond uniformly, caudal neutrophils respond heterogeneously. Collectively, our results reveal that co-existing neutrophils populations with ontogenically distinct origin display functional differences.

Original languageEnglish
Article number4942
JournalNature Communications
Volume14
Issue number1
DOIs
Publication statusPublished - Dec 2023

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology
  • General Physics and Astronomy

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