O-glycosylated cell wall proteins are essential in root hair growth

Silvia M. Velasquez; Martiniano M. Ricardi; Javier Gloazzo Dorosz; Paula V. Fernandez; Alejandro D. Nadra; Laercio Pol-Fachin; Jack Egelund; Sascha Gille; Jesper Harholt; Marina Ciancia; Hugo Verli; Markus Pauly; Antony Bacic; Carl Erik Olsen; Peter Ulvskov; Bent Larsen Petersen; Chris Somerville; Norberto D. Iusem; Jose M. Estevez

doi:10.1126/science.1206657

O-glycosylated cell wall proteins are essential in root hair growth

Silvia M. Velasquez, Martiniano M. Ricardi, Javier Gloazzo Dorosz, Paula V. Fernandez, Alejandro D. Nadra, Laercio Pol-Fachin, Jack Egelund, Sascha Gille, Jesper Harholt, Marina Ciancia, Hugo Verli, Markus Pauly, Antony Bacic, Carl Erik Olsen, Peter Ulvskov, Bent Larsen Petersen, Chris Somerville, Norberto D. Iusem, Jose M. Estevez

Research output: Contribution to journal › Article › peer-review

244 Citations (Scopus)

Abstract

Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

Original language	English
Pages (from-to)	1401-1403
Number of pages	3
Journal	Science
Volume	332
Issue number	6036
DOIs	https://doi.org/10.1126/science.1206657
Publication status	Published - 17 Jun 2011
Externally published	Yes

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Velasquez, S. M., Ricardi, M. M., Dorosz, J. G., Fernandez, P. V., Nadra, A. D., Pol-Fachin, L., Egelund, J., Gille, S., Harholt, J., Ciancia, M., Verli, H., Pauly, M., Bacic, A., Olsen, C. E., Ulvskov, P., Petersen, B. L., Somerville, C., Iusem, N. D., & Estevez, J. M. (2011). O-glycosylated cell wall proteins are essential in root hair growth. Science, 332(6036), 1401-1403. https://doi.org/10.1126/science.1206657

@article{c1ed557d93fc4c1f94ae80d82aeafa5d,

title = "O-glycosylated cell wall proteins are essential in root hair growth",

abstract = "Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.",

author = "Velasquez, {Silvia M.} and Ricardi, {Martiniano M.} and Dorosz, {Javier Gloazzo} and Fernandez, {Paula V.} and Nadra, {Alejandro D.} and Laercio Pol-Fachin and Jack Egelund and Sascha Gille and Jesper Harholt and Marina Ciancia and Hugo Verli and Markus Pauly and Antony Bacic and Olsen, {Carl Erik} and Peter Ulvskov and Petersen, {Bent Larsen} and Chris Somerville and Iusem, {Norberto D.} and Estevez, {Jose M.}",

year = "2011",

month = jun,

day = "17",

doi = "10.1126/science.1206657",

language = "English",

volume = "332",

pages = "1401--1403",

journal = "Science",

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Velasquez, SM, Ricardi, MM, Dorosz, JG, Fernandez, PV, Nadra, AD, Pol-Fachin, L, Egelund, J, Gille, S, Harholt, J, Ciancia, M, Verli, H, Pauly, M, Bacic, A, Olsen, CE, Ulvskov, P, Petersen, BL, Somerville, C, Iusem, ND & Estevez, JM 2011, 'O-glycosylated cell wall proteins are essential in root hair growth', Science, vol. 332, no. 6036, pp. 1401-1403. https://doi.org/10.1126/science.1206657

TY - JOUR

T1 - O-glycosylated cell wall proteins are essential in root hair growth

AU - Velasquez, Silvia M.

AU - Ricardi, Martiniano M.

AU - Dorosz, Javier Gloazzo

AU - Fernandez, Paula V.

AU - Nadra, Alejandro D.

AU - Pol-Fachin, Laercio

AU - Egelund, Jack

AU - Gille, Sascha

AU - Harholt, Jesper

AU - Ciancia, Marina

AU - Verli, Hugo

AU - Pauly, Markus

AU - Bacic, Antony

AU - Olsen, Carl Erik

AU - Ulvskov, Peter

AU - Petersen, Bent Larsen

AU - Somerville, Chris

AU - Iusem, Norberto D.

AU - Estevez, Jose M.

PY - 2011/6/17

Y1 - 2011/6/17

N2 - Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

AB - Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana.

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VL - 332

SP - 1401

EP - 1403

JO - Science

JF - Science

IS - 6036

ER -

O-glycosylated cell wall proteins are essential in root hair growth

Abstract

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