Nutritional status modulates plasma leptin, AMPK and TOR activation, and mitochondrial biogenesis: Implications for cell metabolism and growth in skeletal muscle of the fine flounder

Eduardo N. Fuentes, Diego Safian, Ingibjörg Eir Einarsdottir, Juan Antonio Valdés, Alvaro A. Elorza, Alfredo Molina, Björn Thrandur Björnsson

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

Insight of how growth and metabolism in skeletal muscle are related is still lacking in early vertebrates. In this context, molecules involved in these processes, such as leptin, AMP-activated protein kinase (AMPK), target of rapamicyn (TOR), peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α, and oxidative phosphorylation complexes (OXPHOS), were assessed in the skeletal muscle of a fish species. Periods of fasting followed by a period of refeeding were implemented, using the fine flounder as a model (Paralichthys adspersus). This species exhibits remarkably slow growth and food intake, which is linked to an inherent growth hormone (GH) resistance and high circulating levels of leptin. Leptin increased during fasting concomitantly with AMPK activation, which was inversely correlated with TOR activation. On the other hand, AMPK was directly correlated with an increase in PGC-1α and OXPHOS complexes contents. Dramatic changes in the activation and content of these molecules were observed during short-term refeeding. Leptin, AMPK activation, and PGC-1α/OXPHOS complexes contents decreased radically; whereas, TOR activation increased significantly. During long-term refeeding these molecules returned to basal levels. These results suggest that there is a relation among these components; thus, during fasting periods ATP-consuming biosynthetic pathways are repressed and alternative sources of ATP/energy are promoted, a phenomenon that is reversed during anabolic periods. These results provide novel insight on the control of metabolism and growth in the skeletal muscle of a non-mammalian species, suggesting that both processes in fish muscle are closely related and coordinated by a subset of common molecules.

Original languageEnglish
Pages (from-to)172-180
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume186
DOIs
Publication statusPublished - 1 Jun 2013

Keywords

  • Cell metabolism
  • Fish
  • Growth
  • Nutritional status
  • Signaling pathways
  • Skeletal muscle

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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