Nucleotide sequence of a yeast tRNAArg3A gene and its transcription in a homologous in vitro system

Jorge Villanueva; Paulina Bull; Pablo Valenzuela; Alejandro Venegas

doi:10.1016/0014-5793(84)80854-6

Nucleotide sequence of a yeast tRNA^Arg_3A gene and its transcription in a homologous in vitro system

Jorge Villanueva, Paulina Bull, Pablo Valenzuela, Alejandro Venegas

Life Sciences School

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Twelve bacterial clones containing complementary sequences to yeast tRNA^Arg₃ were isolated from a gene library. The size of the yeast BamHI inserts ranges from 5.4 to 10 MDa. There are at least 6 copies of this gene in different loci of the yeast genome. Insert from clone pYAT-3 was mapped, and the presence of a tRNA^Arg_3A gene was confirmed by DNA sequence. The coding region is colinear with the transcriptional product. Unlike other reported tRAN^Arg_3A genes, this one is not linked to a tRNA^Asp gene. In vitro transcription using a yeast extract produces a transcript of 76 ± 1 bases.

Original language	English
Pages (from-to)	165-169
Number of pages	5
Journal	FEBS Letters
Volume	167
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(84)80854-6
Publication status	Published - 13 Feb 1984

Keywords

DNA sequence
Homologous in vitro transcription
Scattered gene arrangement
Yeast tRNA gene

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(84)80854-6

Cite this

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abstract = "Twelve bacterial clones containing complementary sequences to yeast tRNAArg3 were isolated from a gene library. The size of the yeast BamHI inserts ranges from 5.4 to 10 MDa. There are at least 6 copies of this gene in different loci of the yeast genome. Insert from clone pYAT-3 was mapped, and the presence of a tRNAArg3A gene was confirmed by DNA sequence. The coding region is colinear with the transcriptional product. Unlike other reported tRANArg3A genes, this one is not linked to a tRNAAsp gene. In vitro transcription using a yeast extract produces a transcript of 76 ± 1 bases.",

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AU - Venegas, Alejandro

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