Nucleotide sequence of a yeast tRNAArg3A gene and its transcription in a homologous in vitro system

Jorge Villanueva, Paulina Bull, Pablo Valenzuela, Alejandro Venegas

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Twelve bacterial clones containing complementary sequences to yeast tRNAArg3 were isolated from a gene library. The size of the yeast BamHI inserts ranges from 5.4 to 10 MDa. There are at least 6 copies of this gene in different loci of the yeast genome. Insert from clone pYAT-3 was mapped, and the presence of a tRNAArg3A gene was confirmed by DNA sequence. The coding region is colinear with the transcriptional product. Unlike other reported tRANArg3A genes, this one is not linked to a tRNAAsp gene. In vitro transcription using a yeast extract produces a transcript of 76 ± 1 bases.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalFEBS Letters
Volume167
Issue number1
DOIs
Publication statusPublished - 13 Feb 1984

Keywords

  • DNA sequence
  • Homologous in vitro transcription
  • Scattered gene arrangement
  • Yeast tRNA gene

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Nucleotide sequence of a yeast tRNA<sup>Arg</sup><sub>3A</sub> gene and its transcription in a homologous in vitro system'. Together they form a unique fingerprint.

  • Cite this