Novel Salmonella Phage, vB_Sen_STGO-35-1, Characterization and Evaluation in Chicken Meat

Dácil Rivera, Andrea I. Moreno-Switt, Thomas G. Denes, Lauren K. Hudson, Tracey L. Peters, Reham Samir, Ramy K. Aziz, Jean Paul Noben, Jeroen Wagemans, Fernando Dueñas

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Salmonellosis is one of the most frequently reported zoonotic foodborne diseases world-wide, and poultry is the most important reservoir of Salmonella enterica serovar Enteritidis. The use of lytic bacteriophages (phages) to reduce foodborne pathogens has emerged as a promising bio-control intervention for Salmonella spp. Here, we describe and evaluate the newly isolated Salmonella phage STGO-35-1, including: (i) genomic and phenotypic characterization, (ii) an analysis of the reduction of Salmonella in chicken meat, and (iii) genome plasticity testing. Phage STGO-35-1 repre-sents an unclassified siphovirus, with a length of 47,483 bp, a G + C content of 46.5%, a headful strategy of packaging, and a virulent lifestyle. Phage STGO-35-1 reduced S. Enteritidis counts in chicken meat by 2.5 orders of magnitude at 4 °C. We identified two receptor-binding proteins with affinity to LPS, and their encoding genes showed plasticity during an exposure assay. Phenotypic, proteomic, and genomic characteristics of STGO-35-1, as well as the Salmonella reduction in chicken meat, support the potential use of STGO-35-1 as a targeted biocontrol agent against S. Enteritidis in chicken meat. Additionally, computational analysis and a short exposure time assay allowed us to predict the plasticity of genes encoding putative receptor-binding proteins.

Original languageEnglish
Article number606
JournalMicroorganisms
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 2022

Keywords

  • Receptor-binding proteins
  • Salmonella Enteritidis
  • Salmonella–phage in food
  • Siphoviral morphotype
  • Siphoviridae

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)
  • Virology

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