Native and chemically modified porin channels from Salmonella typhi Ty2 in planar lipid bilayers

Pedro Labarca, Sergio Lobos, Inés Calderón, Guido Mora

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Native porins, from Salmonella typhi Ty2 outer membrane, and porins alkylated with pyridoxal phosphate (Plp) were studied in planar lipid bilayers. The conductance of bilayers exposed to native or chemically modified porins increases in discrete jumps. Conductance histograms for native porins displayed two major peaks at 1.7 and 6.7 nS (in 0.5 M KCl). On the other hand, Plp-treated porins exhibited a single major peak at 1 nS. The relation between bilayer conductance and native porin concentration was linear. However, this relation became logarithmic in the presence of modified porins. The results support the notion that alkaline reduction of S. typhi Ty2 porins with Plp dissociates porin channel trimers in a reversible fashion.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalFEBS Letters
Volume197
Issue number1-2
DOIs
Publication statusPublished - 3 Mar 1986

Keywords

  • (Salmonella typhi Ty2) Porin Pyridoxal phosphate Planar bilayer

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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