Mutaciones del gen supresor de tumor p53 en adenocarcinoma gástrico

Translated title of the contribution: Mutations of p53 suppressor gene in gastric adenocarcinoma

Marcela Aranda L, Neptalí Náquira V, Rafael Karque O, Hegaly Mendoza V, Carolina Sepúlveda T, Claudio Silva Z

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Background: We have shown numeric alterations such as byperploidy and bypoploidy with loss of chromosome 17 in primary gastric cancer. This chromosome maps p53 suppressor gene that induces the transcription of genes related to cellular cycle control, DNA synthesis and repair, cellular differentiation and apoptosis. Aim: To analyze, at a molecular level, the possible alterations of p53 suppressor gene in samples of gastric cancer and non tumoral mucosa. Material and methods: Tissue samples of gastric carcinoma and non tumoral gastric mucosa coming from 26 patients subjected to a total gastrectomy were analyzed. The mutation of p53 suppressor gene exons 7 to 9 were determined using a conformational polymorphism analysis in single strands of the gene and indirect sequencing in some cases. Results: Alterations in p53 gene were found in 77% of tumoral and 19% of non tumoral samples. T insertions in codons 260, 317 and 321, G insertion in codon 328 and G-T transvertion in codon 302 were found. Aminoacid sequence analysis of p53 protein obtained with sequencing data showed that T insertion in codon 260 could translate three erroneous aminoacids after the mutation and produce a truncated protein due to the creation of a stop codon. No associations between alterations of p53 gene and clinical or pathological variables such as age, sex, tumor localization, histological type and presence of lymph node metastases were observed. Conclusions: Mutations of p53 suppressor gene are frequent in gastric carcinoma.

Translated title of the contributionMutations of p53 suppressor gene in gastric adenocarcinoma
Original languageSpanish
Pages (from-to)525-532
Number of pages8
JournalRevista Medica de Chile
Volume126
Issue number5
Publication statusPublished - 1 May 1998

ASJC Scopus subject areas

  • Medicine(all)

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