MOLECULAR PROBES FOR THE IDENTIFICATION AND QUANTITATION OF MICROORGANISMS FOUND IN MINES AND MINE TAILINGS.

James R. Yates, David S. Holmes

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Citations (Scopus)

Abstract

A technique is described for the identification and enumeration of bioleaching microorganisms that circumvents many of the classic problems. The technique is based on the utilization of cloned Thiobacillus ferrooxidans gene sequences as probes for determining genetic homologies using dot blot and Southern blot assays. The results can be quantitated, yielding data on the number as well as the identity of microorganisms. The technique may also prove useful for analyzing samples from bioleaching operations, evaluating the maintenance of genetically-engineered strains in a bioleaching operation and for classifying microorganisms according to their genetic, rather than phenotypic, similarities.

Original languageEnglish
Title of host publicationBiotechnology and Bioengineering Symposium
PublisherJohn Wiley & Sons
Pages301-309
Number of pages9
Edition16
ISBN (Print)0471851027
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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