A technique is described for the identification and enumeration of bioleaching microorganisms that circumvents many of the classic problems. The technique is based on the utilization of cloned Thiobacillus ferrooxidans gene sequences as probes for determining genetic homologies using dot blot and Southern blot assays. The results can be quantitated, yielding data on the number as well as the identity of microorganisms. The technique may also prove useful for analyzing samples from bioleaching operations, evaluating the maintenance of genetically-engineered strains in a bioleaching operation and for classifying microorganisms according to their genetic, rather than phenotypic, similarities.
|Title of host publication||Biotechnology and Bioengineering Symposium|
|Publisher||John Wiley & Sons|
|Number of pages||9|
|Publication status||Published - 1986|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)