TY - JOUR
T1 - Molecular link between auxin and ROS-mediated polar growth
AU - Mangano, Silvina
AU - Denita-Juarez, Silvina Paola
AU - Choi, Hee Seung
AU - Marzol, Eliana
AU - Hwang, Youra
AU - Ranocha, Philippe
AU - Velasquez, Silvia Melina
AU - Borassi, Cecilia
AU - Barberini, María Laura
AU - Aptekmann, Ariel Alejandro
AU - Muschietti, Jorge Prometeo
AU - Nadra, Alejandro Daniel
AU - Dunand, Christophe
AU - Cho, Hyung Taeg
AU - Estevez, José Manuel
N1 - Funding Information:
This work was supported by grants from ANPCyT (PICT2013-003, PICT2014-504, and ICGEB2016; to J.M.E.) and the Mid- Career Researcher Program (2015002633) of the National Research Foundation and the Next-Generation BioGreen 21 Program (Agricultural Genome Center PJ011195) of the Rural Development Administration (to H.-T.C.). H.- S.C. was partially supported by the Stadelmann-Lee Scholarship, Seoul National University.
PY - 2017/5/16
Y1 - 2017/5/16
N2 - Root hair polar growth is endogenously controlled by auxin and sustained by oscillating levels of reactive oxygen species (ROS). These cells extend several hundred-fold their original size toward signals important for plant survival. Although their final cell size is of fundamental importance, the molecular mechanisms that control it remain largely unknown. Here we show that ROS production is controlled by the transcription factor RSL4, which in turn is transcriptionally regulated by auxin through several auxin response factors (ARFs). In this manner, auxin controls ROS-mediated polar growth by activating RSL4, which then up-regulates the expression of genes encoding NADPH oxidases (also known as RESPIRATORY BURST OXIDASE HOMOLOG proteins) and class III peroxidases, which catalyze ROS production. Chemical or genetic interference with ROS balance or peroxidase activity affects root hair final cell size. Overall, our findings establish amolecular link between auxin and ROS-mediated polar root hair growth.
AB - Root hair polar growth is endogenously controlled by auxin and sustained by oscillating levels of reactive oxygen species (ROS). These cells extend several hundred-fold their original size toward signals important for plant survival. Although their final cell size is of fundamental importance, the molecular mechanisms that control it remain largely unknown. Here we show that ROS production is controlled by the transcription factor RSL4, which in turn is transcriptionally regulated by auxin through several auxin response factors (ARFs). In this manner, auxin controls ROS-mediated polar growth by activating RSL4, which then up-regulates the expression of genes encoding NADPH oxidases (also known as RESPIRATORY BURST OXIDASE HOMOLOG proteins) and class III peroxidases, which catalyze ROS production. Chemical or genetic interference with ROS balance or peroxidase activity affects root hair final cell size. Overall, our findings establish amolecular link between auxin and ROS-mediated polar root hair growth.
KW - Auxin
KW - Peroxidases
KW - Root hair growth
KW - ROS
KW - RSL4
UR - http://www.scopus.com/inward/record.url?scp=85019850935&partnerID=8YFLogxK
U2 - 10.1073/pnas.1701536114
DO - 10.1073/pnas.1701536114
M3 - Article
C2 - 28461488
AN - SCOPUS:85019850935
SN - 0027-8424
VL - 114
SP - 5289
EP - 5294
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 20
ER -