TY - JOUR
T1 - Molecular determinants for cyclo-oligosaccharide-based nanoparticle-mediated effective siRNA transfection
AU - Manzanares, Darío
AU - Araya-Durán, Ingrid
AU - Gallego-Yerga, Laura
AU - Játiva, Pablo
AU - Márquez-Miranda, Valeria
AU - Canan, Jonathan
AU - Jiménez Blanco, José Luis
AU - Mellet, Carmen Ortiz
AU - González-Nilo, Fernando Danilo
AU - García Fernández, José Manuel
AU - Ceña, Valentín
N1 - Publisher Copyright:
© 2017 Future Medicine Ltd.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Aim: To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector. Materials & methods: siRNA protection from degradation by RNAses, transfection efficiency and the thermodynamic parameters of the nanoparticle/siRNA interactions were studied on pairs of amphiphilic molecules using biochemical techniques and molecular dynamics. Results: The lower the siRNA solvent accessible surface area in the presence of the nanoparticle, higher the protection from RNAse-mediated degradation in the corresponding nanocomplex; a moderate nanoparticle/siRNA binding energy value further facilitates reversible complexation and binding to the target cellular mRNA. Conclusion: The use, in advance, of these parameters will provide a useful indication of the potential of a molecular nanoparticle as siRNA transfecting vector.
AB - Aim: To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector. Materials & methods: siRNA protection from degradation by RNAses, transfection efficiency and the thermodynamic parameters of the nanoparticle/siRNA interactions were studied on pairs of amphiphilic molecules using biochemical techniques and molecular dynamics. Results: The lower the siRNA solvent accessible surface area in the presence of the nanoparticle, higher the protection from RNAse-mediated degradation in the corresponding nanocomplex; a moderate nanoparticle/siRNA binding energy value further facilitates reversible complexation and binding to the target cellular mRNA. Conclusion: The use, in advance, of these parameters will provide a useful indication of the potential of a molecular nanoparticle as siRNA transfecting vector.
KW - endosomal escape
KW - molecular modeling
KW - molecular nanoparticle
KW - protection from RNAses
KW - protein knock down
KW - siRNA transfection
UR - http://www.scopus.com/inward/record.url?scp=85021648638&partnerID=8YFLogxK
U2 - 10.2217/nnm-2017-0123
DO - 10.2217/nnm-2017-0123
M3 - Article
AN - SCOPUS:85021648638
SN - 1743-5889
VL - 12
SP - 1607
EP - 1621
JO - Nanomedicine
JF - Nanomedicine
IS - 13
ER -