Molecular determinants for cyclo-oligosaccharide-based nanoparticle-mediated effective siRNA transfection

Darío Manzanares, Ingrid Araya-Durán, Laura Gallego-Yerga, Pablo Játiva, Valeria Márquez-Miranda, Jonathan Canan, José Luis Jiménez Blanco, Carmen Ortiz Mellet, Fernando Danilo González-Nilo, José Manuel García Fernández, Valentín Ceña

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Aim: To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector. Materials & methods: siRNA protection from degradation by RNAses, transfection efficiency and the thermodynamic parameters of the nanoparticle/siRNA interactions were studied on pairs of amphiphilic molecules using biochemical techniques and molecular dynamics. Results: The lower the siRNA solvent accessible surface area in the presence of the nanoparticle, higher the protection from RNAse-mediated degradation in the corresponding nanocomplex; a moderate nanoparticle/siRNA binding energy value further facilitates reversible complexation and binding to the target cellular mRNA. Conclusion: The use, in advance, of these parameters will provide a useful indication of the potential of a molecular nanoparticle as siRNA transfecting vector.

Original languageEnglish
Pages (from-to)1607-1621
Number of pages15
JournalNanomedicine
Volume12
Issue number13
DOIs
Publication statusPublished - 1 Jul 2017

Keywords

  • endosomal escape
  • molecular modeling
  • molecular nanoparticle
  • protection from RNAses
  • protein knock down
  • siRNA transfection

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Bioengineering
  • Biomedical Engineering
  • Development
  • General Materials Science

Fingerprint

Dive into the research topics of 'Molecular determinants for cyclo-oligosaccharide-based nanoparticle-mediated effective siRNA transfection'. Together they form a unique fingerprint.

Cite this