Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe

Ira Schwartz, Robin Ann Klotsky, Dirk Elseviers, Patricia J. Gallagher, Manuel Krauskopf, M. A Q Siddiqui, James F H Wong, Bruce A. Roe

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A recombinant plasmid (designated pID2) carrying the E. coli gene for tRNAPhe has been isolated from a plasmid bank constructed by the ligation of a total EcoRI digest of E. coli K12 DNA into the EcoRI site of pACYC184 DNA. The plasmid was selected by virtue of its ability to complement a temperature-sensitive lesion in the gene (PheS) for the α-subunit of phenylalanyl-tRNA synthetase. Crude tRNA isolated from such transformants exhibited elevated levels of phenylalanine acceptor activity. The tRNAPhe gene has been localized within the first 300 base pairs of a 3.6 kb SalI fragment of pID2. The sequence of the gene and its flanking regions is presented.

Original languageEnglish
Pages (from-to)4379-4389
Number of pages11
JournalNucleic Acids Research
Volume11
Issue number13
DOIs
Publication statusPublished - 11 Jul 1983

ASJC Scopus subject areas

  • Genetics

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