Molecular cloning and physical map of bacteriophage PM2 DNA

Alicia Alonso; Angélica Medina; Rafael Vicuña; Alejandro Venegas; Pablo Valenzuela; Arturo Yudelevich

doi:10.1016/0378-1119(81)90049-4

Molecular cloning and physical map of bacteriophage PM2 DNA

Alicia Alonso, Angélica Medina, Rafael Vicuña, Alejandro Venegas, Pablo Valenzuela, Arturo Yudelevich

Life Sciences School

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The entire genome and the DNA fragments of the lipid-containing bacteriophage pM2 were cloned in the pBR322 plasmid vector. A physical map including the sites for the following restriction enzymes was obtained: HpaII, HaeIII, TthI, Sau96I, AvaII, PstI, BstNI, AccI, HincII, HpaI and HindIII. No restriction sites on PM2 DNA were found for BalI, BamHI, BclI, BglI, BglII, BstEII, KpnI, PvuII, SacI, SalI, Sau3A, XbaI and XhoI.

Original language	English
Pages (from-to)	115-118
Number of pages	4
Journal	Gene
Volume	13
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(81)90049-4
Publication status	Published - 1981

Keywords

Alteromonas espejiana
pER322 plasmid vector
Recombinant DNA
restriction site mapping

ASJC Scopus subject areas

Genetics
Medicine(all)

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10.1016/0378-1119(81)90049-4

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title = "Molecular cloning and physical map of bacteriophage PM2 DNA",

abstract = "The entire genome and the DNA fragments of the lipid-containing bacteriophage pM2 were cloned in the pBR322 plasmid vector. A physical map including the sites for the following restriction enzymes was obtained: HpaII, HaeIII, TthI, Sau96I, AvaII, PstI, BstNI, AccI, HincII, HpaI and HindIII. No restriction sites on PM2 DNA were found for BalI, BamHI, BclI, BglI, BglII, BstEII, KpnI, PvuII, SacI, SalI, Sau3A, XbaI and XhoI.",

keywords = "Alteromonas espejiana, pER322 plasmid vector, Recombinant DNA, restriction site mapping",

author = "Alicia Alonso and Ang{\'e}lica Medina and Rafael Vicu{\~n}a and Alejandro Venegas and Pablo Valenzuela and Arturo Yudelevich",

year = "1981",

doi = "10.1016/0378-1119(81)90049-4",

language = "English",

volume = "13",

pages = "115--118",

journal = "Gene",

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T1 - Molecular cloning and physical map of bacteriophage PM2 DNA

AU - Alonso, Alicia

AU - Medina, Angélica

AU - Vicuña, Rafael

AU - Venegas, Alejandro

AU - Valenzuela, Pablo

AU - Yudelevich, Arturo

PY - 1981

Y1 - 1981

N2 - The entire genome and the DNA fragments of the lipid-containing bacteriophage pM2 were cloned in the pBR322 plasmid vector. A physical map including the sites for the following restriction enzymes was obtained: HpaII, HaeIII, TthI, Sau96I, AvaII, PstI, BstNI, AccI, HincII, HpaI and HindIII. No restriction sites on PM2 DNA were found for BalI, BamHI, BclI, BglI, BglII, BstEII, KpnI, PvuII, SacI, SalI, Sau3A, XbaI and XhoI.

AB - The entire genome and the DNA fragments of the lipid-containing bacteriophage pM2 were cloned in the pBR322 plasmid vector. A physical map including the sites for the following restriction enzymes was obtained: HpaII, HaeIII, TthI, Sau96I, AvaII, PstI, BstNI, AccI, HincII, HpaI and HindIII. No restriction sites on PM2 DNA were found for BalI, BamHI, BclI, BglI, BglII, BstEII, KpnI, PvuII, SacI, SalI, Sau3A, XbaI and XhoI.

KW - Alteromonas espejiana

KW - pER322 plasmid vector

KW - Recombinant DNA

KW - restriction site mapping

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DO - 10.1016/0378-1119(81)90049-4

M3 - Article

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AN - SCOPUS:0019499077

VL - 13

SP - 115

EP - 118

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -

Molecular cloning and physical map of bacteriophage PM2 DNA

Abstract

Keywords

ASJC Scopus subject areas

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