Molecular cloning and physical map of bacteriophage PM2 DNA

Alicia Alonso, Angélica Medina, Rafael Vicuña, Alejandro Venegas, Pablo Valenzuela, Arturo Yudelevich

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The entire genome and the DNA fragments of the lipid-containing bacteriophage pM2 were cloned in the pBR322 plasmid vector. A physical map including the sites for the following restriction enzymes was obtained: HpaII, HaeIII, TthI, Sau96I, AvaII, PstI, BstNI, AccI, HincII, HpaI and HindIII. No restriction sites on PM2 DNA were found for BalI, BamHI, BclI, BglI, BglII, BstEII, KpnI, PvuII, SacI, SalI, Sau3A, XbaI and XhoI.

Original languageEnglish
Pages (from-to)115-118
Number of pages4
JournalGene
Volume13
Issue number1
DOIs
Publication statusPublished - 1981

Keywords

  • Alteromonas espejiana
  • pER322 plasmid vector
  • Recombinant DNA
  • restriction site mapping

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

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