TY - JOUR
T1 - Microplastic ingestion cause intestinal lesions in the intertidal fish Girella laevifrons
AU - Ahrendt, C.
AU - Perez-Venegas, D. J.
AU - Urbina, M.
AU - Gonzalez, C.
AU - Echeveste, P.
AU - Aldana, M.
AU - Pulgar, J.
AU - Galbán-Malagón, C.
N1 - Funding Information:
Acknowledgments are given to Ivan Contreras and Carol Stoore from the pathology laboratory due to their help during the histological preparations. The support granted by the Instituto Antártico Chileno and the Armada de Chile for providing Internet connection is gratefully acknowledged. CG-M received financial support from CONICYT - FONDECYT grants 11150548 and 1161504 , PCI REDI170292 , and REDI170403 and INACH RT_12_17 . JP received financial support from CONICYT - FONDECYT grant 1171056 and DI 17- 10R , DI 16-12/R , and DI-495-14/R . DPV acknowledges the doctoral grant supported by the Dirección de Investigación y Doctorados from Universidad Andres Bello . M. Urbina received financial support from CONICYT - FONDECYT grant 11160019 .
PY - 2019/1/1
Y1 - 2019/1/1
N2 - We exposed juvenile intertidal fish to different amounts of Poly(styrene-co-divinylbenzene) microplastics in their diet. We fed ten individuals with pellets containing 0.01 g, another ten fish with pellets containing 0.1 g of PS, and ten fish without plastic as control. After 45 days of treatment, the whole intestine was removed, and the histological evaluation started immediately. We evaluated inflammation due to leukocyte infiltration (Lk), circulatory disorders like Hypermeia (Hyp), and regressive changes in the intestinal tissue, assessing Crypt cell loss (Ccl) and Villi cell loss (Vcl). The severity of the lesions increased according to the microplastic concentration. In the fish group feeding on microplastics, we found that leukocyte infiltration and hyperemia were more severe in the higher exposure group compared to the lower exposure; and crypt cell loss and villi cell loss increased significantly due to Poly(styrene-co-divinylbenzene) microplastic physical abrasion.
AB - We exposed juvenile intertidal fish to different amounts of Poly(styrene-co-divinylbenzene) microplastics in their diet. We fed ten individuals with pellets containing 0.01 g, another ten fish with pellets containing 0.1 g of PS, and ten fish without plastic as control. After 45 days of treatment, the whole intestine was removed, and the histological evaluation started immediately. We evaluated inflammation due to leukocyte infiltration (Lk), circulatory disorders like Hypermeia (Hyp), and regressive changes in the intestinal tissue, assessing Crypt cell loss (Ccl) and Villi cell loss (Vcl). The severity of the lesions increased according to the microplastic concentration. In the fish group feeding on microplastics, we found that leukocyte infiltration and hyperemia were more severe in the higher exposure group compared to the lower exposure; and crypt cell loss and villi cell loss increased significantly due to Poly(styrene-co-divinylbenzene) microplastic physical abrasion.
KW - Coastal
KW - Damage
KW - Intestine
KW - Microplastic exposure
KW - Polystyrene
UR - http://www.scopus.com/inward/record.url?scp=85076623375&partnerID=8YFLogxK
U2 - 10.1016/j.marpolbul.2019.110795
DO - 10.1016/j.marpolbul.2019.110795
M3 - Article
AN - SCOPUS:85076623375
SN - 0025-326X
VL - 151
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
M1 - 110795
ER -