Manganese affects the production of laccase in the basidiomycete Ceriporiopsis subvermispora

Augusto Manubens, Paulo Canessa, Carolina Folch, Marcela Avila, Loreto Salas, Rafael Vicuña

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35 Citations (Scopus)


The authors have previously identified and characterized lcs, a gene encoding laccase in the white-rot basidiomycete Ceriporiopsis subvermispora. In this work, the effect of Mn2+ in the production of extracellular laccase in liquid cultures of this fungus has been assessed. It was observed that at low (0-10 μM) concentrations of Mn2+, high titers of lcs-mRNA were obtained, whereas at high (160-194 μM) concentrations of this metal ion, transcripts levels decreased markedly. This phenomenon was observed at different days of growth. On the other hand, Cu2+ or Ag +, but not Zn2+ or Cd2+, led to an accumulation of lcs transcripts only in cultures grown in the absence of Mn2+. A dramatic increase in lcs transcript levels was also obtained with syringic acid, a lignin-related aromatic compound. This effect was more pronounced in cultures lacking Mn2+. In the course of these studies it was observed that Mn2+ stimulates mycelium growth. Thus, although extracellular laccase activity appeared higher in cultures containing 160 or 194 μM Mn 2+, i.e. when lcs transcripts were lower, a correlation between lcs-mRNA levels and enzymatic activity was observed when values of the latter were corrected by the amount of mycelium present in the cultures.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - Oct 2007


  • Laccase
  • Ligninolytic
  • Manganese
  • Metal regulation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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