Laminin inhibits amyloid-β-peptide fibrillation

Francisca C. Bronfman; Jorge Garrido; Alejandra Alvarez; Carlos Morgan; Nibaldo C. Inestrosa

doi:10.1016/S0304-3940(96)13147-5

Laminin inhibits amyloid-β-peptide fibrillation

Francisca C. Bronfman, Jorge Garrido, Alejandra Alvarez, Carlos Morgan, Nibaldo C. Inestrosa

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

Laminin, an important extracellular matrix component is induced by brain injury and colocalizes with amyloid-β-peptide (Aβ) deposits in Alzheimer brains. We report here that laminin inhibits amyloid fibril formation as determined by thioflavin T fluorescence spectroscopy and electron microscopic examination. The inhibition of amyloid formation by laminin was concentration dependent and was observed at a laminin concentration of 300 nM, corresponding to a laminin/Aβ protein molar ratio of 1:800. The potential effect of laminin, may prove important to inhibit Aβ fibrillogenesis in vivo, specifically at the level of cerebral blood vessels.

Original language	English
Pages (from-to)	201-203
Number of pages	3
Journal	Neuroscience Letters
Volume	218
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(96)13147-5
Publication status	Published - 8 Nov 1996
Externally published	Yes

Keywords

Alzheimer's disease
Amyloid-β-peptide
Laminin

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0304-3940(96)13147-5

Cite this

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title = "Laminin inhibits amyloid-β-peptide fibrillation",

abstract = "Laminin, an important extracellular matrix component is induced by brain injury and colocalizes with amyloid-β-peptide (Aβ) deposits in Alzheimer brains. We report here that laminin inhibits amyloid fibril formation as determined by thioflavin T fluorescence spectroscopy and electron microscopic examination. The inhibition of amyloid formation by laminin was concentration dependent and was observed at a laminin concentration of 300 nM, corresponding to a laminin/Aβ protein molar ratio of 1:800. The potential effect of laminin, may prove important to inhibit Aβ fibrillogenesis in vivo, specifically at the level of cerebral blood vessels.",

keywords = "Alzheimer's disease, Amyloid-β-peptide, Laminin",

author = "Bronfman, {Francisca C.} and Jorge Garrido and Alejandra Alvarez and Carlos Morgan and Inestrosa, {Nibaldo C.}",

note = "Funding Information: We thank Dr. Mauricio Boric for sharing with us his Shimadzu spectrofluorometer. Mr. Jos6 Morillas for technical assistance with the electron microscope and photography. This work was supported by Predoctoral Fellowships from CONICYT to F.C.B. and FONDECYT (Grant 1940694) and a Presidential Chair in Science from the Chilean Government to N.C.I.",

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doi = "10.1016/S0304-3940(96)13147-5",

language = "English",

volume = "218",

pages = "201--203",

journal = "Neuroscience Letters",

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T1 - Laminin inhibits amyloid-β-peptide fibrillation

AU - Bronfman, Francisca C.

AU - Garrido, Jorge

AU - Alvarez, Alejandra

AU - Morgan, Carlos

AU - Inestrosa, Nibaldo C.

N1 - Funding Information: We thank Dr. Mauricio Boric for sharing with us his Shimadzu spectrofluorometer. Mr. Jos6 Morillas for technical assistance with the electron microscope and photography. This work was supported by Predoctoral Fellowships from CONICYT to F.C.B. and FONDECYT (Grant 1940694) and a Presidential Chair in Science from the Chilean Government to N.C.I.

PY - 1996/11/8

Y1 - 1996/11/8

N2 - Laminin, an important extracellular matrix component is induced by brain injury and colocalizes with amyloid-β-peptide (Aβ) deposits in Alzheimer brains. We report here that laminin inhibits amyloid fibril formation as determined by thioflavin T fluorescence spectroscopy and electron microscopic examination. The inhibition of amyloid formation by laminin was concentration dependent and was observed at a laminin concentration of 300 nM, corresponding to a laminin/Aβ protein molar ratio of 1:800. The potential effect of laminin, may prove important to inhibit Aβ fibrillogenesis in vivo, specifically at the level of cerebral blood vessels.

AB - Laminin, an important extracellular matrix component is induced by brain injury and colocalizes with amyloid-β-peptide (Aβ) deposits in Alzheimer brains. We report here that laminin inhibits amyloid fibril formation as determined by thioflavin T fluorescence spectroscopy and electron microscopic examination. The inhibition of amyloid formation by laminin was concentration dependent and was observed at a laminin concentration of 300 nM, corresponding to a laminin/Aβ protein molar ratio of 1:800. The potential effect of laminin, may prove important to inhibit Aβ fibrillogenesis in vivo, specifically at the level of cerebral blood vessels.

KW - Alzheimer's disease

KW - Amyloid-β-peptide

KW - Laminin

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U2 - 10.1016/S0304-3940(96)13147-5

DO - 10.1016/S0304-3940(96)13147-5

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SN - 0304-3940

VL - 218

SP - 201

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JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

Laminin inhibits amyloid-β-peptide fibrillation

Abstract

Keywords

ASJC Scopus subject areas

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