Kinetic characterization of calcium uptake by the rat liver Golgi Apparatus

Patricio Rojas; Amarilys Surroca; Ariel Orellana; Daniel Wolff

doi:10.1006/cbir.2000.0496

Kinetic characterization of calcium uptake by the rat liver Golgi Apparatus

Patricio Rojas, Amarilys Surroca, Ariel Orellana, Daniel Wolff

Life Sciences School

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23 Citations (Scopus)

Abstract

We carried out a kinetic characterization of the Ca²⁺ active transport in the rat liver Golgi Apparatus (GA) membrane. Calcium accumulation by vesicles of a GA enriched fraction was found to be a function of both Ca²⁺ and ATP-Mg concentrations, it was inhibited by 2 μM thapsigargin but not stimulated by 3 μM calmodulin. The kinetic parameter values obtained for the GA Ca²⁺ pump were: J(max) of 3.96 nmol/mg min, K(m) for Ca²⁺ of 0.150 μM and two K(m)'s for ATP of 1.14 μM and 519 μM. These results were almost identical to those obtained for the endoplasmic reticulum (ER) fraction, indicating that the GA Ca²⁺ pump is a sarco/endoplasmic reticulum (SERCA) P-type, analogous if not identical to that present in the ER. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	229-233
Number of pages	5
Journal	Cell Biology International
Volume	24
Issue number	4
DOIs	https://doi.org/10.1006/cbir.2000.0496
Publication status	Published - 2000

Keywords

Calcium stores
Golgi apparatus
Rat liver
Serca calcium pump

ASJC Scopus subject areas

Cell Biology

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10.1006/cbir.2000.0496

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abstract = "We carried out a kinetic characterization of the Ca2+ active transport in the rat liver Golgi Apparatus (GA) membrane. Calcium accumulation by vesicles of a GA enriched fraction was found to be a function of both Ca2+ and ATP-Mg concentrations, it was inhibited by 2 μM thapsigargin but not stimulated by 3 μM calmodulin. The kinetic parameter values obtained for the GA Ca2+ pump were: J(max) of 3.96 nmol/mg min, K(m) for Ca2+ of 0.150 μM and two K(m)'s for ATP of 1.14 μM and 519 μM. These results were almost identical to those obtained for the endoplasmic reticulum (ER) fraction, indicating that the GA Ca2+ pump is a sarco/endoplasmic reticulum (SERCA) P-type, analogous if not identical to that present in the ER. (C) 2000 Academic Press.",

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T1 - Kinetic characterization of calcium uptake by the rat liver Golgi Apparatus

AU - Rojas, Patricio

AU - Surroca, Amarilys

AU - Orellana, Ariel

AU - Wolff, Daniel

PY - 2000

Y1 - 2000

N2 - We carried out a kinetic characterization of the Ca2+ active transport in the rat liver Golgi Apparatus (GA) membrane. Calcium accumulation by vesicles of a GA enriched fraction was found to be a function of both Ca2+ and ATP-Mg concentrations, it was inhibited by 2 μM thapsigargin but not stimulated by 3 μM calmodulin. The kinetic parameter values obtained for the GA Ca2+ pump were: J(max) of 3.96 nmol/mg min, K(m) for Ca2+ of 0.150 μM and two K(m)'s for ATP of 1.14 μM and 519 μM. These results were almost identical to those obtained for the endoplasmic reticulum (ER) fraction, indicating that the GA Ca2+ pump is a sarco/endoplasmic reticulum (SERCA) P-type, analogous if not identical to that present in the ER. (C) 2000 Academic Press.

AB - We carried out a kinetic characterization of the Ca2+ active transport in the rat liver Golgi Apparatus (GA) membrane. Calcium accumulation by vesicles of a GA enriched fraction was found to be a function of both Ca2+ and ATP-Mg concentrations, it was inhibited by 2 μM thapsigargin but not stimulated by 3 μM calmodulin. The kinetic parameter values obtained for the GA Ca2+ pump were: J(max) of 3.96 nmol/mg min, K(m) for Ca2+ of 0.150 μM and two K(m)'s for ATP of 1.14 μM and 519 μM. These results were almost identical to those obtained for the endoplasmic reticulum (ER) fraction, indicating that the GA Ca2+ pump is a sarco/endoplasmic reticulum (SERCA) P-type, analogous if not identical to that present in the ER. (C) 2000 Academic Press.

KW - Calcium stores

KW - Golgi apparatus

KW - Rat liver

KW - Serca calcium pump

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JO - Cell Biology International

JF - Cell Biology International

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ER -

Kinetic characterization of calcium uptake by the rat liver Golgi Apparatus

Abstract

Keywords

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