Kinetic characterization of calcium uptake by the rat liver Golgi Apparatus

Patricio Rojas, Amarilys Surroca, Ariel Orellana, Daniel Wolff

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22 Citations (Scopus)

Abstract

We carried out a kinetic characterization of the Ca2+ active transport in the rat liver Golgi Apparatus (GA) membrane. Calcium accumulation by vesicles of a GA enriched fraction was found to be a function of both Ca2+ and ATP-Mg concentrations, it was inhibited by 2 μM thapsigargin but not stimulated by 3 μM calmodulin. The kinetic parameter values obtained for the GA Ca2+ pump were: J(max) of 3.96 nmol/mg min, K(m) for Ca2+ of 0.150 μM and two K(m)'s for ATP of 1.14 μM and 519 μM. These results were almost identical to those obtained for the endoplasmic reticulum (ER) fraction, indicating that the GA Ca2+ pump is a sarco/endoplasmic reticulum (SERCA) P-type, analogous if not identical to that present in the ER. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)229-233
Number of pages5
JournalCell Biology International
Volume24
Issue number4
DOIs
Publication statusPublished - 1 Jan 2000

Keywords

  • Calcium stores
  • Golgi apparatus
  • Rat liver
  • Serca calcium pump

ASJC Scopus subject areas

  • Cell Biology

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