IST2: An insertion sequence from Thiobacillus ferrooxidans

J. R. Yates, R. P. Cunningham, D. S. Holmes

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The genome of Thiobacillus ferrooxidans (strain ATCC 19859) contains at least two families of repeated sequences, termed family 1 and 2. The nucleotide sequence of a family 2 member was determined. It is 1408 base pairs long and has structural features similar to those of insertion sequences (IS elements). Terminal inverted repeats 25 base pairs in length are present. These inverted repeats are imperfect and adjacent to target-site duplications 9 base pairs in length. Several open reading frames were detected (the longest was 888 base pairs). We have named this IS element-like sequence IST2. The ends of a second example of IST2 were analyzed and compared to those of the first. The DNA sequences are identical and similarly sized target-site duplications are present.

Original languageEnglish
Pages (from-to)7284-7287
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number19
DOIs
Publication statusPublished - 1988

ASJC Scopus subject areas

  • General

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