TY - JOUR
T1 - Intra-articular treatment with corticosteroids increases apoptosis in human rotator cuff tears
AU - Ramírez, Juan Pablo
AU - Bonati-Richardson, Francesca
AU - García, María Pía
AU - Hidalgo, Christian
AU - Stoore, Caroll
AU - Liendo, Rodrigo
AU - Soza, Francisco
AU - Landerer, Eduardo
AU - Paredes, Rodolfo
N1 - Funding Information:
This work was supported by the FONDECYT-Chile: [Grant number 1161475] and Universidad Andres Bello-Chile: [Grant number DI 56-11/R].
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Purpose: The aim of this study is to evaluate in vivo the level of apoptosis in human rotator cuff tears and the relationship it might have with tendon degeneration. Methods: Rotator cuff biopsies from 19 male and female patients, ages between 38 and 68 years, with and without previous corticosteroid infiltrations were collected via arthroscopy. Biopsies from seven patients with healthy rotator cuffs were used as a control group. An in situ terminal deoxynucleotidyl transferase dUTP nick end labeling assay was performed to detect the level of apoptosis, which was expressed as a percentage of apoptotic cells (PAC). Results: PAC in patients with corticosteroid infiltrations was 76.97 ± 16.99 in all tendon rupture zones, in non-infiltrated patients was 35.89 ± 22.96, whereas in control patients was 14.48 ± 8.15. Likewise, the tendency of PAC reveals that apoptosis in control and non-infiltrated groups was different and dispersed in all tear zones; while in corticosteroid treated patients, the tendency was similar in all rupture sites. Conclusions: This investigation leads us to conclude that the administration of corticosteroid is associated with a higher amount of apoptosis at the insertion site of the rotator cuff (rupture edge).
AB - Purpose: The aim of this study is to evaluate in vivo the level of apoptosis in human rotator cuff tears and the relationship it might have with tendon degeneration. Methods: Rotator cuff biopsies from 19 male and female patients, ages between 38 and 68 years, with and without previous corticosteroid infiltrations were collected via arthroscopy. Biopsies from seven patients with healthy rotator cuffs were used as a control group. An in situ terminal deoxynucleotidyl transferase dUTP nick end labeling assay was performed to detect the level of apoptosis, which was expressed as a percentage of apoptotic cells (PAC). Results: PAC in patients with corticosteroid infiltrations was 76.97 ± 16.99 in all tendon rupture zones, in non-infiltrated patients was 35.89 ± 22.96, whereas in control patients was 14.48 ± 8.15. Likewise, the tendency of PAC reveals that apoptosis in control and non-infiltrated groups was different and dispersed in all tear zones; while in corticosteroid treated patients, the tendency was similar in all rupture sites. Conclusions: This investigation leads us to conclude that the administration of corticosteroid is associated with a higher amount of apoptosis at the insertion site of the rotator cuff (rupture edge).
KW - Apoptosis
KW - corticosteroid infiltration
KW - rotator cuff
UR - http://www.scopus.com/inward/record.url?scp=85052087976&partnerID=8YFLogxK
U2 - 10.1080/03008207.2018.1501040
DO - 10.1080/03008207.2018.1501040
M3 - Article
AN - SCOPUS:85052087976
SN - 0300-8207
VL - 60
SP - 283
EP - 290
JO - Connective Tissue Research
JF - Connective Tissue Research
IS - 3
ER -
