In situ nuclear organization of regulatory machinery

Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. Van Wijnen, Jane B. Lian, Janet L. Stein, Gary S. Stein

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Original languageEnglish
Title of host publicationOsteoporosis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Number of pages21
ISBN (Print)9781588298287
Publication statusPublished - 2008

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cell synchrony
  • Confocal microscopy
  • FRAP
  • Immunofluorescence microscopy
  • Live cell microscopy
  • Nuclear matrix
  • Nuclear organization
  • Runx

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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