In situ nuclear organization of regulatory machinery.

Shirwin M. Pockwinse; Sayyed K. Zaidi; Ricardo F. Medina; Rachit Bakshi; Krishna P. Kota; Syed A. Ali; Daniel W. Young; Jeffery A. Nickerson; Amjad Javed; Martin Montecino; Andre J. van Wijnen; Jane B. Lian; Janet L. Stein; Gary S. Stein

In situ nuclear organization of regulatory machinery.

Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, Gary S. Stein

Life Sciences School

Research output: Contribution to journal › Article › peer-review

Abstract

Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Original language	English
Pages (from-to)	239-259
Number of pages	21
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	455
Publication status	Published - 2008

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

@article{826170988e4f42d193d8212441fc0b81,

title = "In situ nuclear organization of regulatory machinery.",

abstract = "Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.",

author = "Pockwinse, {Shirwin M.} and Zaidi, {Sayyed K.} and Medina, {Ricardo F.} and Rachit Bakshi and Kota, {Krishna P.} and Ali, {Syed A.} and Young, {Daniel W.} and Nickerson, {Jeffery A.} and Amjad Javed and Martin Montecino and {van Wijnen}, {Andre J.} and Lian, {Jane B.} and Stein, {Janet L.} and Stein, {Gary S.}",

year = "2008",

language = "English",

volume = "455",

pages = "239--259",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - In situ nuclear organization of regulatory machinery.

AU - Pockwinse, Shirwin M.

AU - Zaidi, Sayyed K.

AU - Medina, Ricardo F.

AU - Bakshi, Rachit

AU - Kota, Krishna P.

AU - Ali, Syed A.

AU - Young, Daniel W.

AU - Nickerson, Jeffery A.

AU - Javed, Amjad

AU - Montecino, Martin

AU - van Wijnen, Andre J.

AU - Lian, Jane B.

AU - Stein, Janet L.

AU - Stein, Gary S.

PY - 2008

Y1 - 2008

N2 - Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

AB - Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

UR - http://www.scopus.com/inward/record.url?scp=45549107608&partnerID=8YFLogxK

M3 - Article

C2 - 18463823

SN - 1064-3745

VL - 455

SP - 239

EP - 259

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

In situ nuclear organization of regulatory machinery.

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this